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  SLC10A4 is a vesicular amine-associated transporter modulating dopamine homeostasis.

Larhammar, M., Patra, K., Blunder, M., Emilsson, L., Peuckert, C., Arvidsson, E., et al. (2015). SLC10A4 is a vesicular amine-associated transporter modulating dopamine homeostasis. Biological Psychatry, 77(6), 526-536. doi:10.1016/j.biopsych.2014.07.017.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-773F-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CC55-8
Genre: Journal Article

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 Creators:
Larhammar, M., Author
Patra, K., Author
Blunder, M., Author
Emilsson, L., Author
Peuckert, C., Author
Arvidsson, E., Author
Ronnlund, D., Author
Preobraschenski, J.1, Author              
Birgner, C., Author
Limbach, C., Author
Widengren, J., Author
Blom, H., Author
Jahn, R.1, Author              
Wallen-Mackenzie, A., Author
Kullander, K., Author
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              

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Free keywords: Acetylcholine; Amphetamine; Central nervous system; Cocaine; Noradrenaline; Serotonin; Synaptic transmission; Transmitter
 Abstract: BACKGROUND: The neuromodulatory transmitters, biogenic amines, have profound effects on multiple neurons and are essential for normal behavior and mental health. Here we report that the orphan transporter SLC10A4, which in the brain is exclusively expressed in presynaptic vesicles of monoaminergic and cholinergic neurons, has a regulatory role in dopamine homeostasis. METHODS: We used a combination of molecular and behavioral analyses, pharmacology, and in vivo amperometry to assess the role of SLC10A4 in dopamine- regulated behaviors. RESULTS: We show that SLC10A4 is localized on the same synaptic vesicles as either vesicular acetylcholine transporter or vesicular monoamine transporter 2. We did not find evidence for direct transport of dopamine by SLC10A4; however, synaptic vesicle preparations lacking SLC10A4 showed decreased dopamine vesicular uptake efficiency. Furthermore, we observed an increased acidification in synaptic vesicles isolated from mice over-expressing SLC10A4. Loss of SLC10A4 in mice resulted in reduced striatal serotonin, noradrenaline, and dopamine concentrations and a significantly higher dopamine turnover ratio. Absence of SLC10A4 led to slower dopamine clearance rates in vivo, which resulted in accumulation of extracellular dopamine. Finally, whereas SLC10A4 null mutant mice were slightly hypoactive, they displayed hypersensitivity to administration of amphetamine and tranylcypromine. CONCLUSIONS: Our results demonstrate that SLC10A4 is a vesicular monoaminergic and cholinergic associated transporter that is important for dopamine homeostasis and neuromodulation in vivo. The discovery of SLC10A4 and its role in dopaminergic signaling reveals a novel mechanism for neuromodulation and represents an unexplored target for the treatment of neurological and mental disorders.

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Language(s): eng - English
 Dates: 2015-03-15
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.biopsych.2014.07.017
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Title: Biological Psychatry
Source Genre: Journal
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Pages: - Volume / Issue: 77 (6) Sequence Number: - Start / End Page: 526 - 536 Identifier: -