English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation.

Schwarz, D., Orf, I., Kopka, J., & Hagemann, M. (2014). Effects of Inorganic Carbon Limitation on the Metabolome of the Synechocystis sp. PCC 6803 Mutant Defective in glnB Encoding the Central Regulator PII of Cyanobacterial C/N Acclimation. Metabolites, 4(2), 232-247. doi:10.3390/metabo4020232.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-789F-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-78A0-7
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Schwarz, Doreen1, Author
Orf, I.2, Author              
Kopka, J.2, Author              
Hagemann, Martin1, Author
Affiliations:
1external, ou_persistent22              
2Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              

Content

show
hide
Free keywords: -
 Abstract: Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Non-diazotrophic strains such as the model Synechocystis sp. PCC 6803 depend on a balanced uptake and assimilation of inorganic carbon and nitrogen sources. The internal C/N ratio is sensed via the PII protein (GlnB). We analyzed metabolic changes of the DglnB mutant of Synechocystis sp. PCC 6803 under different CO2 availability. The identified metabolites provided a snapshot of the central C/N metabolism. Cells of the DglnB mutant shifted to carbon-limiting conditions, i.e. a decreased C/N ratio, showed changes in intermediates of the sugar storage and particularly of the tricarboxylic acid cycle, arginine, and glutamate metabolism. The changes of the metabolome support the notion that the PII protein is primarily regulating the N-metabolism whereas the changes in C-metabolism are probably secondary effects of the PII deletion.

Details

show
hide
Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 24957024
DOI: 10.3390/metabo4020232
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Metabolites
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: MDPI AG
Pages: - Volume / Issue: 4 (2) Sequence Number: - Start / End Page: 232 - 247 Identifier: Other: 2218-1989
CoNE: https://pure.mpg.de/cone/journals/resource/2218-1989