ausblenden:
Schlagwörter:
-
Zusammenfassung:
For reconstruction of controlled cell division in
a minimal
cell
model, or protocell
,
a positioning mechanism that spatially regulates division is indispensable. In
Escherichia coli
,
the Min protein
s oscillate from pole to pole to determine the division site by inhibition of
the
primary
divisome protein FtsZ anywhere but in the cell middle. Remarkably, when
reconstituted under defined conditions
in vitro
, the Min proteins self
-
organize into
spatiotem
poral patterns in the presence of a
lipid
membrane and ATP. We review recent
progress made in studying the Min system
in vitro
,
particularly focusing on
the effect
s
of
various
physicochemical parameters and boundary conditions
on pattern formation
.
Furthermore
, we
discuss implications and challenges for utilizing the Min system for
division site placement in
proto
cells.
