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  A new probe for super-resolution imaging of membranes elucidates trafficking pathways.

Revelo, N. H., Kamin, D., Truckenbrodt, S., Wong, A. B., Reuter-Jessen, K., Reisinger, E., et al. (2014). A new probe for super-resolution imaging of membranes elucidates trafficking pathways. Journal of Cell Biology, 205(4), 591-606. doi:10.1083/jcb.201402066.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-A918-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CCC3-1
Genre: Journal Article

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 Creators:
Revelo, N. H., Author
Kamin, D.1, Author              
Truckenbrodt, S., Author
Wong, A. B., Author
Reuter-Jessen, K., Author
Reisinger, E., Author
Moser, T., Author
Rizzoli, S. O., Author
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: The molecular composition of the organelles involved in membrane recycling is difficult to establish as a result of the absence of suitable labeling tools. We introduce in this paper a novel probe, named membrane-binding fluorophore-cysteine-lysine-palmitoyl group (mCLING), which labels the plasma membrane and is taken up during endocytosis. It remains attached to membranes after fixation and permeabilization and can therefore be used in combination with immunostaining and super-resolution microscopy. We applied mCLING to mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue. mCLING enabled us to study the molecular composition of different trafficking organelles. We used it to address several questions related to synaptic vesicle recycling in the auditory inner hair cells from the organ of Corti and to investigate molecular differences between synaptic vesicles that recycle actively or spontaneously in cultured neurons. We conclude that mCLING enables the investigation of trafficking membranes in a broad range of preparations.

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Language(s): eng - English
 Dates: 2014-05-26
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1083/jcb.201402066
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Title: Journal of Cell Biology
Source Genre: Journal
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Pages: - Volume / Issue: 205 (4) Sequence Number: - Start / End Page: 591 - 606 Identifier: -