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  How to Measure Molecular Forces in Cells: A Guide to Evaluating Genetically-Encoded FRET-Based Tension Sensors

Cost, A.-L., Ringer, P., Chrostek-Grashoff, A., & Grashoff, C. (2015). How to Measure Molecular Forces in Cells: A Guide to Evaluating Genetically-Encoded FRET-Based Tension Sensors. Cellular and Molecular Bioengineering, 8(1), 96-105. doi:10.1007/s12195-014-0368-1.

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art%3A10.1007%2Fs12195-014-0368-1.pdf (Any fulltext), 844KB
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 Creators:
Cost, Anna-Lena1, Author           
Ringer, Pia1, Author           
Chrostek-Grashoff, Anna1, Author           
Grashoff, Carsten1, Author           
Affiliations:
1Grashoff, Carsten / Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565150              

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Free keywords: Mechanobiology, Förster resonance energy transfer, Biosensors, Mechanotransduction, Fluorescence lifetime imaging microscopy.
 Abstract: The ability of cells to sense and respond to mechanical forces is central to a wide range of biological processes and plays an important role in numerous pathol- ogies. The molecular mechanisms underlying cellular mech- anotransduction, however, have remained largely elusive because suitable methods to investigate subcellular force propagation were missing. Here, we review recent advances in the development of biosensors that allow molecular force measurements. We describe the underlying principle of currently available techniques and propose a strategy to systematically evaluate new Fo ̈ rster resonance energy trans- fer (FRET)-based biosensors

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Language(s): eng - English
 Dates: 2014-122015-03
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s12195-014-0368-1
 Degree: -

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Title: Cellular and Molecular Bioengineering
Source Genre: Journal
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Publ. Info: New York : Springer
Pages: - Volume / Issue: 8 (1) Sequence Number: - Start / End Page: 96 - 105 Identifier: ISSN: 1865-5033 (online)
ISSN: 1865-5025 (print)