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Free keywords:
Ataxia Telangiectasia Mutated Proteins/genetics/metabolism
Cell Line
Epigenesis, Genetic/*radiation effects
Gene Expression Profiling/methods
Gene Regulatory Networks/*radiation effects
Humans
*Radiation, Ionizing
Transcriptome/*radiation effects
Tumor Suppressor Protein p53/genetics/metabolism
Abstract:
The DNA damage response (DDR) is a vast signaling network that is robustly activated by DNA double-strand breaks, the critical lesion induced by ionizing radiation (IR). Although much of this response operates at the protein level, a critical component of the network sustains many DDR branches by modulating the cellular transcriptome. Using deep sequencing, we delineated three layers in the transcriptional response to IR in human breast cancer cells: changes in the expression of genes encoding proteins or long noncoding RNAs, alterations in genomic binding by key transcription factors, and dynamics of epigenetic markers of active promoters and enhancers. We identified protein-coding and previously unidentified noncoding genes that were responsive to IR, and demonstrated that IR-induced transcriptional dynamics was mediated largely by the transcription factors p53 and nuclear factor kappaB (NF-kappaB) and was primarily dependent on the kinase ataxia-telangiectasia mutated (ATM). The resultant data set provides a rich resource for understanding a basic, underlying component of a critical cellular stress response.
