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  Isolation of clathrin-coated vesicles from tissue culture cells.

Borner, G. H. H., & Fielding, A. B. (2014). Isolation of clathrin-coated vesicles from tissue culture cells. Cold Spring Harbor protocols, 2014(11), 1136-1138. doi:10.1101/pdb.top074435.

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 Creators:
Borner, Georg H. H.1, Author              
Fielding, Andrew B2, Author
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

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 Abstract: The study of clathrin-coated vesicles (CCVs) isolated from various organs has revealed the identities and important features of many of the factors involved in membrane trafficking. The development of isolation methods using cultured cell lines has made it possible to manipulate the source material before isolation to ask important questions about the roles of these factors and the pathways in which they are involved. We discuss here the advantages and limitations of the use of cultured cell lines for the isolation of CCVs. 2014 Cold Spring Harbor Laboratory Press.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: ISI: 25368317
DOI: 10.1101/pdb.top074435
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Title: Cold Spring Harbor protocols
Source Genre: Journal
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Pages: - Volume / Issue: 2014 (11) Sequence Number: - Start / End Page: 1136 - 1138 Identifier: ISSN: 1559-6095