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  DNA damage focus analysis in blood samples of minipigs reveals acute partial body irradiation

Lamkowski, A., Forcheron, F., Agay, D., Ahmed, E. A., Drouet, M., Meineke, V., et al. (2014). DNA damage focus analysis in blood samples of minipigs reveals acute partial body irradiation. PLoS One, 9(2): e87458. doi:10.1371/journal.pone.0087458.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-C270-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-C271-2
Genre: Journal Article

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© 2014 Lamkowski et al
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 Creators:
Lamkowski, A., Author
Forcheron, F., Author
Agay, D., Author
Ahmed, E. A., Author
Drouet, M., Author
Meineke, V., Author
Scherthan, H.1, 2, Author              
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              
2Institut für Radiobiologie der Bundeswehr in Verb. mit der Universität Ulm, München, Germany, ou_persistent22              

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Free keywords: Animals Cell Nucleus/genetics/metabolism/radiation effects DNA Breaks, Double-Stranded/radiation effects *DNA Damage DNA Repair Enzymes/metabolism DNA-Binding Proteins/metabolism Dose-Response Relationship, Radiation Female Histones/*metabolism Immunohistochemistry Intracellular Signaling Peptides and Proteins/metabolism Lymphocytes/metabolism/radiation effects Microscopy, Fluorescence Phosphorylation/radiation effects *Radiation, Ionizing Radiometry/methods Reproducibility of Results Swine Swine, Miniature
 Abstract: Radiation accidents frequently involve acute high dose partial body irradiation leading to victims with radiation sickness and cutaneous radiation syndrome that implements radiation-induced cell death. Cells that are not lethally hit seek to repair ionizing radiation (IR) induced damage, albeit at the expense of an increased risk of mutation and tumor formation due to misrepair of IR-induced DNA double strand breaks (DSBs). The response to DNA damage includes phosphorylation of histone H2AX in the vicinity of DSBs, creating foci in the nucleus whose enumeration can serve as a radiation biodosimeter. Here, we investigated gammaH2AX and DNA repair foci in peripheral blood lymphocytes of Gottingen minipigs that experienced acute partial body irradiation (PBI) with 49 Gy (+/- 6%) Co-60 gamma-rays of the upper lumbar region. Blood samples taken 4, 24 and 168 hours post PBI were subjected to gamma-H2AX, 53BP1 and MRE11 focus enumeration. Peripheral blood lymphocytes (PBL) of 49 Gy partial body irradiated minipigs were found to display 1-8 DNA damage foci/cell. These PBL values significantly deceed the high foci numbers observed in keratinocyte nuclei of the directly gamma-irradiated minipig skin regions, indicating a limited resident time of PBL in the exposed tissue volume. Nonetheless, PBL samples obtained 4 h post IR in average contained 2.2% of cells displaying a pan-gammaH2AX signal, suggesting that these received a higher IR dose. Moreover, dispersion analysis indicated partial body irradiation for all 13 minipigs at 4 h post IR. While dose reconstruction using gammaH2AX DNA repair foci in lymphocytes after in vivo PBI represents a challenge, the DNA damage focus assay may serve as a rapid, first line indicator of radiation exposure. The occurrence of PBLs with pan-gammaH2AX staining and of cells with relatively high foci numbers that skew a Poisson distribution may be taken as indicator of acute high dose partial body irradiation, particularly when samples are available early after IR exposure.

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Language(s): eng - English
 Dates: 2014-02-03
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1371/journal.pone.0087458
ISSN: 1932-6203 (Electronic)
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 9 (2) Sequence Number: e87458 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850