English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Contrasting behavior of heterochromatic and euchromatic chromosome portions and pericentric genome separation in pre-bouquet spermatocytes of hybrid mice

Scherthan, H., Schöfisch, K., Dell, T., & Illner, D. (2014). Contrasting behavior of heterochromatic and euchromatic chromosome portions and pericentric genome separation in pre-bouquet spermatocytes of hybrid mice. Chromosoma: Biology of the Nucleus, 123(6), 609-624. doi:10.1007/s00412-014-0479-4.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-C0B1-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-C0B2-F
Genre: Journal Article

Files

show Files
hide Files
:
Scherthan.pdf (Publisher version), 6MB
Name:
Scherthan.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© 2014 Springer International Publishing AG, Part of Springer Science+Business Media
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Scherthan, H.1, 2, Author              
Schöfisch, K.3, Author              
Dell, T., Author
Illner, D., Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              
2Institut für Radiobiologie der Bundeswehr in Verb. mit der Univ. Ulm, 80937, München, Germany , ou_persistent22              
3Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

Content

show
hide
Free keywords: -
 Abstract: The spatial distribution of parental genomes has attracted much interest because intranuclear chromosome distribution can modulate the transcriptome of cells and influence the efficacy of meiotic homologue pairing. Pairing of parental chromosomes is imperative to sexual reproduction as it translates into homologue segregation and genome haploidization to counteract the genome doubling at fertilization. Differential FISH tagging of parental pericentromeric genome portions and specific painting of euchromatic chromosome arms in Mus musculus (MMU) x Mus spretus (MSP) hybrid spermatogenesis disclosed a phase of homotypic non-homologous pericentromere clustering that led to parental pericentric genome separation from the pre-leptoteneup to zygotene stages. Preferential clustering of MMU pericentromeres correlated with particular enrichment of epigenetic marks (H3K9me3), HP1-gamma and structural maintenance of chromosomes SMC6 complex proteins at the MMU major satellite DNA repeats. In contrast to the separation of heterochromatic pericentric genome portions, the euchromatic arms of homeologous chromosomes showed considerable presynaptic pairing already during leptotene stage of all mice investigated. Pericentric genome separation was eventually disbanded by telomere clustering that concentrated both parental pericentric genome portions in a limited nuclear sector of the bouquet nucleus. Our data disclose the differential behavior of pericentromeric heterochromatin and the euchromatic portions of the parental genomes during homologue search. Homotypic pericentromere clustering early in prophase I may contribute to the exclusion of large repetitive DNA domains from homology search, while the telomere bouquet congregates and registers spatially separated portions of the genome to fuel synapsis initiation and high levels of homologue pairing, thus contributing to the fidelity of meiosis and reproduction.

Details

show
hide
Language(s): eng - English
 Dates: 2014-08-152014-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s00412-014-0479-4
ISSN: 1432-0886 (Electronic)0009-5915 (Print)
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Chromosoma : Biology of the Nucleus
  Other : Chromosoma
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Wien : Springer-Verlag
Pages: - Volume / Issue: 123 (6) Sequence Number: - Start / End Page: 609 - 624 Identifier: ISSN: 0009-5915
CoNE: https://pure.mpg.de/cone/journals/resource/991042749609288