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  mFISH analysis of chromosome aberrations in workers occupationally exposed to mixed radiation

Sotnik, N. V., Osovets, S. V., Schertan, H., & Azizova, T. V. (2014). mFISH analysis of chromosome aberrations in workers occupationally exposed to mixed radiation. Radiation and Environmental Biophysics, 53(2), 347-354. doi:10.1007/s00411-014-0536-7.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-C096-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-C09C-3
Genre: Journal Article

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© 2014 Springer International Publishing AG, Part of Springer Science+Business Media
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Sotnik, N. V., Author
Osovets, S. V., Author
Schertan, H.1, 2, Author              
Azizova, T. V., Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              
2Bundeswehr Institute of Radiobiology Affiliated to the University of Ulm, Neuherbergstr. 11, 80937, Munich, Germany , ou_persistent22              

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Free keywords: Chromosome Aberrations/radiation effects Color Female Humans In Situ Hybridization, Fluorescence/*methods Male Nuclear Reactors Occupational Exposure/*adverse effects/*analysis Radiometry/*methods Russia Young Adult
 Abstract: We performed a study on the presence of chromosome aberrations in a cohort of plutonium workers of the Mayak production association (PA) with a mean age of 73.3 +/- 7.2 years to see whether by multi-color fluorescence in situ hybridization (mFISH) translocation analysis can discriminate individuals who underwent occupational exposure with internal and/or external exposure to ionizing radiation 40 years ago. All Mayak PA workers were occupationally exposed to chronic internal alpha-radiation due to incorporated plutonium-239 and/or to external gamma-rays. First, we obtained the translocation yield in control individuals by mFISH to chromosome spreads of age-matched individuals and obtained background values that are similar to previously published values of an international study (Sigurdson et al. in Mutat Res 652:112-121, 2008). Workers who had absorbed a total dose of >0.5 Gy external gamma-rays to the red bone marrow (RBM) displayed a significantly higher frequency of stable chromosome aberrations relative to a group of workers exposed to <0.5 Gy gamma-rays total absorbed RBM dose. Thus, the translocation frequency may be considered to be a biological marker of external radiation exposure even years after the exposure. In a group of workers who were internally exposed and had incorporated plutonium-239 at a body burden >1.48 kBq, mFISH revealed a considerable number of cells with complex chromosomal rearrangements. Linear associations were observed for translocation yield with the absorbed RBM dose from external gamma-rays as well as for complex chromosomal rearrangements with the plutonium-239 body burden.

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Language(s): eng - English
 Dates: 2014-04-092014-05
 Publication Status: Published in print
 Pages: -
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 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s00411-014-0536-7
ISSN: 1432-2099 (Electronic)0301-634X (Print)
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Title: Radiation and Environmental Biophysics
  Other : Radiat. Environ. Biophys.
Source Genre: Journal
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Publ. Info: Berlin : Springer-Verlag
Pages: - Volume / Issue: 53 (2) Sequence Number: - Start / End Page: 347 - 354 Identifier: ISSN: 0301-634X
CoNE: https://pure.mpg.de/cone/journals/resource/954925510402