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  Single muscle fiber proteomics reveals unexpected mitochondrial specialization.

Murgia, M., Nagaraj, N., Deshmukh, A. S., Zeiler, M., Cancellara, P., Moretti, I., et al. (2015). Single muscle fiber proteomics reveals unexpected mitochondrial specialization. EMBO reports, 16(3), 387-395. doi:10.15252/embr.201439757.

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 Creators:
Murgia, Marta1, Author           
Nagaraj, Nagarjuna1, Author           
Deshmukh, Atul S.1, Author           
Zeiler, Marlis1, Author           
Cancellara, Pasqua2, Author
Moretti, Irene2, Author
Reggiani, Carlo2, Author
Schiaffino, Stefano2, Author
Mann, Matthias1, Author           
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1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

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 Abstract: Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: ISI: 25643707
DOI: 10.15252/embr.201439757
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Title: EMBO reports
Source Genre: Journal
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Pages: - Volume / Issue: 16 (3) Sequence Number: - Start / End Page: 387 - 395 Identifier: -