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  SRF is essential for mesodermal cell migration during elongation of the embryonic body axis

Schwartz, B., Marks, M., Wittler, L., Werber, M., Währisch, S., Nordheim, A., et al. (2014). SRF is essential for mesodermal cell migration during elongation of the embryonic body axis. Mechanisms of Development, 133, 23-35. doi:10.1016/j.mod.2014.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-A823-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-A824-9
Genre: Journal Article

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 Creators:
Schwartz, Benedikt1, Author              
Marks, Matthias1, Author              
Wittler, Lars1, Author              
Werber, Martin1, Author              
Währisch, Sandra1, Author              
Nordheim, Alfred, Author
Herrmann, Bernhard G.1, Author              
Grote, Phillip1, Author              
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

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Free keywords: Serum Response Factor (SRF); Brachyury; Mouse embryo; Body axis elongation; Migration; Epithelial–mesenchymal transition
 Abstract: Mesoderm formation in the mouse embryo initiates around E6.5 at the primitive streak and continues until the end of axis extension at E12.5. It requires the process of epithelial-to-mesenchymal transition (EMT), wherein cells detach from the epithelium, adopt mesenchymal cell morphology, and gain competence to migrate. It was shown previously that, prior to mesoderm formation, the transcription factor SRF (Serum Response Factor) is essential for the formation of the primitive streak. To elucidate the role of murine Srf in mesoderm formation during axis extension we conditionally inactivated Srf in nascent mesoderm using the T(s)::Cre driver mouse. Defects in mutant embryos became apparent at E8.75 in the heart and in the allantois. From E9.0 onwards body axis elongation was arrested. Using genome-wide expression analysis, combined with SRF occupancy data from ChIP-seq analysis, we identified a set of direct SRF target genes acting in posterior nascent mesoderm which are enriched for transcripts associated with migratory function. We further show that cell migration is impaired in Srf mutant embryos. Thus, the primary role for SRF in the nascent mesoderm during elongation of the embryonic body axis is the activation of a migratory program, which is a prerequisite for axis extension.

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Language(s): eng - English
 Dates: 2014-07-112014-08
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.mod.2014
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Title: Mechanisms of Development
Source Genre: Journal
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Publ. Info: Shannon : Elsevier
Pages: - Volume / Issue: 133 Sequence Number: - Start / End Page: 23 - 35 Identifier: ISSN: 0925-4773
CoNE: /journals/resource/954927703126