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  Tyrosine phosphorylation of RNA polymerase II CTD is associated with antisense promoter transcription and active enhancers in mammalian cells

Descostes, N., Heidemann, M., Spinelli, L., Schüller, R., Maqbool, M. A., Fenouil, R., et al. (2014). Tyrosine phosphorylation of RNA polymerase II CTD is associated with antisense promoter transcription and active enhancers in mammalian cells. eLife, 3: e02105. doi:10.7554/eLife.02105.

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Descostes, Nicolas, Author
Heidemann, Martin, Author
Spinelli, Lionel, Author
Schüller, Roland, Author
Maqbool, Muhammad Ahmad, Author
Fenouil, Romain, Author
Koch, Frederic1, Author              
Innocenti, Charlène, Author
Gut, Marta, Author
Gut, Ivo, Author
Eick, Dirk, Author
Andrau, Jean-Christophe, Author
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1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

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 Abstract: In mammals, the carboxy-terminal domain (CTD) of RNA polymerase (Pol) II consists of 52 conserved heptapeptide repeats containing the consensus sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Post-translational modifications of the CTD coordinate the transcription cycle and various steps of mRNA maturation. Here we describe Tyr1 phosphorylation (Tyr1P) as a hallmark of promoter (5′ associated) Pol II in mammalian cells, in contrast to what was described in yeast. Tyr1P is predominantly found in antisense orientation at promoters but is also specifically enriched at active enhancers. Mutation of Tyr1 to phenylalanine (Y1F) prevents the formation of the hyper-phosphorylated Pol IIO form, induces degradation of Pol II to the truncated Pol IIB form, and results in a lethal phenotype. Our results suggest that Tyr1P has evolved specialized and essential functions in higher eukaryotes associated with antisense promoter and enhancer transcription, and Pol II stability.

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Language(s): eng - English
 Dates: 2014-05-09
 Publication Status: Published online
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.7554/eLife.02105
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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 3 Sequence Number: e02105 Start / End Page: - Identifier: Other: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X