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  Embryonic development and maternal regulation of murine circadian clock function.

Landgraf, D., Achten, C., Dallmann, F., & Oster, H. (2015). Embryonic development and maternal regulation of murine circadian clock function. Chronobiology International, 32(3), 416-427. doi:10.3109/07420528.2014.986576.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-B046-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-F621-B
Genre: Journal Article

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Landgraf, D.1, Author              
Achten, C., Author
Dallmann, F., Author
Oster, H.1, Author              
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1Research Group of Circadian Rhythms, MPI for biophysical chemistry, Max Planck Society, ou_578594              

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 Abstract: The importance of circadian clocks in the regulation of adult physiology in mammals is well established. In contrast, the ontogenesis of the circadian system and its role in embryonic development are still poorly understood. Although there is experimental evidence that the clock machinery is present prior to birth, data on gestational clock functionality are inconsistent. Moreover, little is known about the dependence of embryonic rhythms on maternal and environmental time cues and the role of circadian oscillations for embryonic development. The aim of this study was to test if fetal mouse tissues from early embryonic stages are capable of expressing endogenous, self-sustained circadian rhythms and their contribution to embryogenesis. Starting on embryonic day 13, we collected precursor tissues for suprachiasmatic nucleus (SCN), liver and kidney from embryos carrying the circadian reporter gene Per2::Luc and investigated rhythmicity and circadian traits of these tissues ex vivo. We found that even before the respective organs were fully developed, embryonic tissues were capable of expressing circadian rhythms. Period and amplitude of which were determined very early during development and phases of liver and kidney explants are not influenced by tissue preparation, whereas SCN explants phasing is strongly dependent on preparation time. Embryonic circadian rhythms also developed in the absence of maternal and environmental time signals. Morphological and histological comparison of offspring from matings of Clock-Delta19 mutant and wild-type mice revealed that both fetal and maternal clocks have distinct roles in embryogenesis. While genetic disruptions of maternal and embryonic clock function leads to increased fetal fat depots, abnormal ossification and organ development, Clock gene mutant newborns from mothers with a functional clock showed a larger body size compared to wild-type littermates. These data may contribute to the understanding of the ontogenesis of circadian clocks and the risk of disturbed maternal or embryonic circadian rhythms for embryonic development.

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Language(s): eng - English
 Dates: 2014-11-282015-04
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.3109/07420528.2014.986576
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Title: Chronobiology International
Source Genre: Journal
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Pages: - Volume / Issue: 32 (3) Sequence Number: - Start / End Page: 416 - 427 Identifier: -