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  Three multihaem cytochromes c from the hyperthermophilic archaeon Ignicoccus hospitalis: purification, properties and localization

Naß, B., Pöll, U., Langer, J. D., Kreuter, L., Küper, U., Flechsler, J., et al. (2014). Three multihaem cytochromes c from the hyperthermophilic archaeon Ignicoccus hospitalis: purification, properties and localization. Microbiology/SGM, 160(6), 1278-1289. doi:10.1099/mic.0.077792-0.

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 Creators:
Naß, Bastian1, Author
Pöll, Uwe1, Author
Langer, Julian David2, Author                 
Kreuter, Lydia3, Author
Küper, Ulf3, Author
Flechsler, Jennifer4, Author
Heimerl, Thomas4, Author
Rachel, Reinhard4, Author
Huber, Harald3, Author
Kletzin, Arnulf1, Author
Affiliations:
1Technische Universität Darmstadt, Microbiology – Sulfur Biochemistry and Microbial Bioenergetics, Darmstadt, Germany, ou_persistent22              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              
3Lehrstuhl für Mikrobiologie, Universität Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany , ou_persistent22              
4Zentrum für Elektronenmikroskopie, Universität Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany , ou_persistent22              

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 Abstract: Three different multihaem cytochromes c were purified from cell extracts of the hyperthermophilic archaeon Ignicoccus hospitalis. One tetrahaem cytochrome, locus tag designation Igni_0530, was purified from membrane fractions together with the iron–sulfur protein Igni_0529. Two octahaem cytochromes, Igni_0955 and Igni_1359, were purified from soluble fractions but were also present in the membrane fraction. N-terminal sequencing showed that three of the four proteins had their signal peptides cleaved off, while results were ambiguous for Igni_0955. In contrast, mass spectrometry of Igni_0955 and Igni_1359 resulted in single mass peaks including the signal sequences and eight haems per subunit and so both forms might be present in the cell. Igni_0955 and Igni_1359 belong to the hydroxylamine dehydrogenase (HAO) family (29% mutual identity). HAO or reductase activities with inorganic sulfur compounds were not detected. Igni_0955 was reduced by enriched I. hospitalis hydrogenase at a specific activity of 243 nmol min-1 (mg hydrogenase)-1 while activity was non-existent for Igni_0530 and low for Igni_1359. Immuno-electron microscopy of ultra-thin sections showed that Igni_0955 and Igni_1359 are located in both I. hospitalis membranes and also in the intermembrane compartment. We concluded that these cytochromes might function as electron shuttles between the hydrogenase in the outer cellular membrane and cellular reductases, whereas Igni_0530 might be part of the sulfur-reducing mechanism.

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Language(s): eng - English
 Dates: 20142014-04-022014-04-042014-06
 Publication Status: Issued
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1099/mic.0.077792-0
 Degree: -

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Title: Microbiology/SGM
Source Genre: Journal
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Publ. Info: Reading, U.K. : Society for General Microbiology
Pages: 12 Volume / Issue: 160 (6) Sequence Number: - Start / End Page: 1278 - 1289 Identifier: ISSN: 1350-0872
CoNE: https://pure.mpg.de/cone/journals/resource/954927546246