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  A vertebrate-conserved cis-regulatory module for targeted expression in the main hypothalamic regulatory region for the stress response

Gutierrez-Triana, J. A., Herget, U., Lichtner, P., Castillo Ramirez, L. A., & Ryu, S. (2014). A vertebrate-conserved cis-regulatory module for targeted expression in the main hypothalamic regulatory region for the stress response. BMC Developmental Biology, 14: 41. doi:10.1186/s12861-014-0041-x.

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Gutierrez-Triana, José Arturo1, Author           
Herget, Ulrich1, Author           
Lichtner, Patrick1, Author           
Castillo Ramirez, Luis Alberto1, Author           
Ryu, Soojin1, Author           
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1Max Planck Research Group Developmental Genetics of the nervous system (Soojin Ryu), Max Planck Institute for Medical Research, Max Planck Society, ou_1497724              

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Free keywords: Otp, Evolutionarily conserved non-coding regions, cis-regulatory module, Hypothalamic-pituitary-adrenal axis, Neurosecretory preoptic area, Cortisol, Zebrafish, Stress response
 Abstract: BackgroundThe homeodomain transcription factor orthopedia (Otp) is an evolutionarily conserved regulator of neuronal fates. In vertebrates, Otp is necessary for the proper development of different regions of the brain and is required in the diencephalon to specify several hypothalamic cell types, including the cells that control the stress response. To understand how this widely expressed transcription factor accomplishes hypothalamus-specific functions, we performed a comprehensive screening of otp cis-regulatory regions in zebrafish.ResultsHere, we report the identification of an evolutionarily conserved vertebrate enhancer module with activity in a restricted area of the forebrain, which includes the region of the hypothalamus that controls the stress response. This region includes neurosecretory cells producing Corticotropin-releasing hormone (Crh), Oxytocin (Oxt) and Arginine vasopressin (Avp), which are key components of the stress axis. Lastly, expression of the bacterial nitroreductase gene under this specific enhancer allowed pharmacological attenuation of the stress response in zebrafish larvae.ConclusionVertebrates share many cellular and molecular components of the stress response and our work identified a striking conservation at the cis-regulatory level of a key hypothalamic developmental gene. In addition, this enhancer provides a useful tool to manipulate and visualize stress-regulatory hypothalamic cells in vivo with the long-term goal of understanding the ontogeny of the stress axis in vertebrates

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Language(s): eng - English
 Dates: 2014-06-102014-11-112014-11-27
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: Other: 8067
DOI: 10.1186/s12861-014-0041-x
 Degree: -

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Title: BMC Developmental Biology
Source Genre: Journal
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Publ. Info: BioMed Central
Pages: - Volume / Issue: 14 Sequence Number: 41 Start / End Page: - Identifier: ISSN: 1471-213X
CoNE: https://pure.mpg.de/cone/journals/resource/111000136905004