English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Composition and variation of the skin microbiota in sympatric species of European newts (Salamandridae)

Vences, M., Granzow, S., Künzel, S., Tebbe, C. C., Baines, J. F., & Dohrmann, A. B. (2015). Composition and variation of the skin microbiota in sympatric species of European newts (Salamandridae). Amphibia Reptilia, 36(1), 5-12. doi:10.1163/15685381-00002970.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-BAE8-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-C170-A
Genre: Journal Article

Files

show Files
hide Files
:
Vences_et_al_2015.pdf (Publisher version), 460KB
 
File Permalink:
-
Name:
Vences_et_al_2015.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Evolutionary Biology, Plön; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Vences, Miguel, Author
Granzow, Susann, Author
Künzel, Sven1, Author              
Tebbe, Christoph C., Author
Baines, John F.2, Author              
Dohrmann, Anja B., Author
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              
2Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445638              

Content

show
hide
Free keywords: Pseudomonas; Flavobacterium; Janthinobacterium; Lissotriton vulgaris; 16S rRNA gene; Betaproteobacteria; Amphibia; Caudata; Ichthyosaura alpestris; Triturus cristatus
 Abstract: The mucous skin of amphibians provides a habitat for microorganisms which may interact with their hosts and thereby affect their condition and health. Cultivation-independent analyses of the bacterial communities based on the detection of PCR-amplified bacterial 16S rRNA genes provides a direct approach to characterize their diversity. In the present pilot study we utilized this approach in combination with a high-throughput DNA sequencing technology (454 pyrosequencing), to characterize the bacterial community structure of the skin of three newt species (Lissotriton vulgaris, Ichthyosaura alpestris, Triturus cristatus), collected near Braunschweig, Germany. 16S rDNA sequences were obtained from 19 unique samples. On average, 6113 amplicon sequences were obtained per sample and these could phylogenetically be assigned to a total of 1615 different operational taxonomic units (OTUs). Altogether, most samples were rather similar in their dominant bacterial taxa. Most represented were Betaproteobacteria (46%; mostly Janthinobacterium), Gammaproteobacteria (28%; mostly Pseudomonas), Flavobacteria (phylum Bacteroidetes: 19%, mostly Flavobacterium), and Sphingobacteria (Bacteroidetes: 5%, mostly Pedobacter). We found no significant differences between the three newt species, or between hemi-nested vs. non-nested PCR, but a strong difference among sampling dates (15 and 17 April 2013) which might be explained by the ongoing transition of the newts from their terrestrial to aquatic phase which coincided with this period, or by differences between sexes as these were unevenly sampled on the two dates. 16S rRNA gene sequences retrieved in this study in several cases were identical or very similar to those previously found on the skin of North American salamanders.

Details

show
hide
Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1163/15685381-00002970
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Amphibia Reptilia
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 36 (1) Sequence Number: - Start / End Page: 5 - 12 Identifier: ISSN: 0173-5373 (print)
ISSN: 1568-5381 (online)