English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Integrated annotation and analysis of in situ hybridization images using the ImAnno system: Application to the ear and sensory organs of the fetal mouse.

Romand, R., Ripp, R., Poidevin, L., Boeglin, M., Geffers, L., Dollé, P., et al. (2015). Integrated annotation and analysis of in situ hybridization images using the ImAnno system: Application to the ear and sensory organs of the fetal mouse. PLOS One, 10(2): e0118024. doi:10.1371/journal.pone.0118024.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-BC8F-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-6EFA-2
Genre: Journal Article

Files

show Files
hide Files
:
2147627.pdf (Publisher version), 9MB
Name:
2147627.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2147627_Suppl_1.TIF (Supplementary material), 473KB
Name:
2147627_Suppl_1.TIF
Description:
-
Visibility:
Public
MIME-Type / Checksum:
image/tiff / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2147627_Suppl_2.XLSX (Supplementary material), 38KB
Name:
2147627_Suppl_2.XLSX
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2147627_Suppl_3.DOCX (Supplementary material), 18KB
Name:
2147627_Suppl_3.DOCX
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2147627_Suppl_4.XLSX (Supplementary material), 11KB
Name:
2147627_Suppl_4.XLSX
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Romand, R., Author
Ripp, R., Author
Poidevin, L., Author
Boeglin, M., Author
Geffers, L.1, Author              
Dollé, P., Author
Poch, O., Author
Affiliations:
1Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society, ou_persistent34              

Content

show
hide
Free keywords: -
 Abstract: An in situ hybridization (ISH) study was performed on 2000murine genes representing around 10% of the protein-coding genes present in the mouse genome using data generated by the EURExpress consortium. This study was carried out in 25 tissues of late gestation embryos (E14.5), with a special emphasis on the developing ear and on five distinct developing sensory organs, including the cochlea, the vestibular receptors, the sensory retina, the olfactory organ, and the vibrissae follicles. The results obtained from an analysis of more than 11,000 micrographs have been integrated in a newly developed knowledgebase, called ImAnno. In addition to managing the multilevel micrograph annotations performed by human experts, ImAnno provides public access to various integrated databases and tools. Thus, it facilitates the analysis of complex ISH gene expression patterns, as well as functional annotation and interaction of gene sets. It also provides direct links to human pathways and diseases. Hierarchical clustering of expression patterns in the 25 tissues revealed three main branches corresponding to tissues with common functions and/or embryonic origins. To illustrate the integrative power of ImAnno, we explored the expression, function and disease traits of the sensory epithelia of the five presumptive sensory organs. The study identified 623 genes (out of 2000) concomitantly expressed in the five embryonic epithelia, among which many (similar to 12%) were involved in human disorders. Finally, various multilevel interaction networks were characterized, highlighting differential functional enrichments of directly or indirectly interacting genes. These analyses exemplify an under-represention of "sensory" functions in the sensory gene set suggests that E14.5 is a pivotal stage between the developmental stage and the functional phase that will be fully reached only after birth.

Details

show
hide
Language(s): eng - English
 Dates: 2015-02-23
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0118024
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLOS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 17 Volume / Issue: 10 (2) Sequence Number: e0118024 Start / End Page: - Identifier: -