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  Primer extension capture: targeted sequence retrieval from heavily degraded DNA sources (Videodocumentation)

Briggs, A. W., Good, J. M., Green, R. E., Krause, J., Maricic, T., Stenzel, U., et al. (2009). Primer extension capture: targeted sequence retrieval from heavily degraded DNA sources (Videodocumentation). Journal of visualized experiments: JoVE, (31): 1573, pp. 1573-1573. doi:10.3791/1573.

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 Urheber:
Briggs, Adrian W, Autor
Good, Jeffrey M, Autor
Green, Richard E, Autor
Krause, Johannes1, Autor           
Maricic, Tomislav, Autor
Stenzel, Udo, Autor
Pääbo, Svante, Autor
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1External Organizations, ou_persistent22              

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 Zusammenfassung: We present a method of targeted DNA sequence retrieval from DNA sources which are heavily degraded and contaminated with microbial DNA, as is typical of ancient bones. The method greatly reduces sample destruction and sequencing demands relative to direct PCR or shotgun sequencing approaches. We used this method to reconstruct the complete mitochondrial DNA (mtDNA) genomes of five Neandertals from across their geographic range. The mtDNA genetic diversity of the late Neandertals was approximately three times lower than that of contemporary modern humans. Together with analyses of mtDNA protein evolution, these data suggest that the long-term effective population size of Neandertals was smaller than that of modern humans and extant great apes.

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Sprache(n): eng - English
 Datum: 2009-09-032009-09-03
 Publikationsstatus: Erschienen
 Seiten: 4
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: Library amplification
Primer Extension
Extension Product Capture
Capture Product Amplification
Representative Results
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.3791/1573
ISI: 19730410
Anderer: shh0026
 Art des Abschluß: -

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Titel: Journal of visualized experiments : JoVE
Genre der Quelle: Zeitschrift
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Seiten: 3 Band / Heft: (31) Artikelnummer: 1573 Start- / Endseite: 1573 - 1573 Identifikator: ISSN: 1940-087X
CoNE: https://pure.mpg.de/cone/journals/resource/1940-087X