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  Carotenoid-chlorophyll coupling and fluorescence quenching in aggregated minor PSII proteins CP24 and CP29.

Holleboom, C. P., Gacek, D. A., Liao, P. N., Negretti, M., Croce, R., & Walla, P. J. (2015). Carotenoid-chlorophyll coupling and fluorescence quenching in aggregated minor PSII proteins CP24 and CP29. Photosynthesis Research, 124(2), 171-180. doi:10.1007/s11120-015-0113-1.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-C3A7-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CD0F-B
Genre: Journal Article

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2149289.pdf (Publisher version), 521KB
 
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 Creators:
Holleboom, C. P., Author
Gacek, D. A., Author
Liao, P. N., Author
Negretti, M., Author
Croce, R., Author
Walla, P. J.1, Author              
Affiliations:
1Research Group of Biomolecular Spectroscopy and Single-Molecule Detection, MPI for biophysical chemistry, Max Planck Society, ou_578565              

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Free keywords: CP24; CP29; LHCII; Aggregation; Electronic coupling; Fluorescence quenching; Chlorophylls; Carotenoids
 Abstract: It is known that aggregation of isolated light-harvesting complex II (LHCII) in solution results in high fluorescence quenching, reduced chlorophyll fluorescence lifetime, and increased electronic coupling of carotenoid (Car) S-1 and chlorophyll (Chl) Q(y) states, as determined by two-photon studies. It has been suggested that this behavior of aggregated LHCII mimics aspects of non-photochemical quenching processes of higher plants and algae. However, several studies proposed that the minor photosystem II proteins CP24 and CP29 also play a significant role in regulation of photosynthesis. Therefore, we use a simple protocol that allows gradual aggregation also of CP24 and CP29. Similarly, as observed for LHCII, aggregation of CP24 and CP29 also leads to increasing fluorescence quenching and increasing electronic Car S-1-Chl Q(y) coupling. Furthermore, a direct comparison of the three proteins revealed a significant higher electronic coupling in the two minor proteins already in the absence of any aggregation. These differences become even more prominent upon aggregation. A red-shift of the Q(y) absorption band known from LHCII aggregation was also observed for CP29 but not for CP24. We discuss possible implications of these results for the role of CP24 and CP29 as potential valves for excess excitation energy in the regulation of photosynthetic light harvesting.

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Language(s): eng - English
 Dates: 2015-03-06
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s11120-015-0113-1
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Title: Photosynthesis Research
Source Genre: Journal
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Pages: - Volume / Issue: 124 (2) Sequence Number: - Start / End Page: 171 - 180 Identifier: -