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  Sampling of glycan-bound conformers by the anti-HIV lectin Oscillatoria agardhii agglutinin in the absence of sugar.

Carneiro, M. G., Koharudin, L. M., Ban, D., Sabo, T. M., Trigo-Mourino, P., Mazur, A., et al. (2015). Sampling of glycan-bound conformers by the anti-HIV lectin Oscillatoria agardhii agglutinin in the absence of sugar. Angewandte Chemie International Edition, 54(22), 6462-6465. doi:10.1002/anie.201500213.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-C6EC-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CDA1-1
Genre: Journal Article

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 Creators:
Carneiro, M. G.1, Author              
Koharudin, L. M., Author
Ban, D.1, Author              
Sabo, T. M.1, Author              
Trigo-Mourino, P.1, Author              
Mazur, A.1, Author              
Griesinger, C.1, Author              
Gronenborn, A. M., Author
Lee, D.1, Author              
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1Department of NMR-based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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Free keywords: NMR spectroscopy; anti-HIV lectins; conformational selection; ground state; proteins
 Abstract: Lectins from different sources have been shown to interfere with HIV infection by binding to the sugars of viral-envelope glycoproteins. Three-dimensional atomic structures of a number of HIV-inactivating lectins have been determined, both as free proteins and in glycan-bound forms. However, details on the mechanism of recognition and binding to sugars are elusive. Herein we focus on the anti-HIV lectin OAA from Oscillatoria agardhii: We show that in the absence of sugars in solution, both the sugar-free and sugar-bound protein conformations that were observed in the X-ray crystal structures exist as conformational substates. Our results suggest that glycan recognition occurs by conformational selection within the ground state; this model differs from the popular "excited-state" model. Our findings provide further insight into molecular recognition of the major receptor on the HIV virus by OAA. These details can potentially be used for the optimization and/or development of preventive anti-HIV therapeutics.

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Language(s): eng - English
 Dates: 2015-04-142015-05-26
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/anie.201500213
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Title: Angewandte Chemie International Edition
Source Genre: Journal
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Pages: - Volume / Issue: 54 (22) Sequence Number: - Start / End Page: 6462 - 6465 Identifier: -