English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Sampling of glycan-bound conformers by the anti-HIV lectin Oscillatoria agardhii agglutinin in the absence of sugar.

Carneiro, M. G., Koharudin, L. M., Ban, D., Sabo, T. M., Trigo-Mourino, P., Mazur, A., et al. (2015). Sampling of glycan-bound conformers by the anti-HIV lectin Oscillatoria agardhii agglutinin in the absence of sugar. Angewandte Chemie International Edition, 54(22), 6462-6465. doi:10.1002/anie.201500213.

Item is

Files

show Files
hide Files
:
2149631.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
2149631.pdf
Description:
-
OA-Status:
Visibility:
Restricted (UNKNOWN id 303; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2149631_Suppl.pdf (Supplementary material), 3MB
Name:
2149631_Suppl.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Carneiro, M. G.1, Author           
Koharudin, L. M., Author
Ban, D.1, Author           
Sabo, T. M.1, Author           
Trigo-Mourino, P.1, Author           
Mazur, A.1, Author           
Griesinger, C.1, Author           
Gronenborn, A. M., Author
Lee, D.1, Author           
Affiliations:
1Department of NMR-based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: NMR spectroscopy; anti-HIV lectins; conformational selection; ground state; proteins
 Abstract: Lectins from different sources have been shown to interfere with HIV infection by binding to the sugars of viral-envelope glycoproteins. Three-dimensional atomic structures of a number of HIV-inactivating lectins have been determined, both as free proteins and in glycan-bound forms. However, details on the mechanism of recognition and binding to sugars are elusive. Herein we focus on the anti-HIV lectin OAA from Oscillatoria agardhii: We show that in the absence of sugars in solution, both the sugar-free and sugar-bound protein conformations that were observed in the X-ray crystal structures exist as conformational substates. Our results suggest that glycan recognition occurs by conformational selection within the ground state; this model differs from the popular "excited-state" model. Our findings provide further insight into molecular recognition of the major receptor on the HIV virus by OAA. These details can potentially be used for the optimization and/or development of preventive anti-HIV therapeutics.

Details

show
hide
Language(s): eng - English
 Dates: 2015-04-142015-05-26
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.201500213
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Angewandte Chemie International Edition
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 54 (22) Sequence Number: - Start / End Page: 6462 - 6465 Identifier: -