English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Enhanced In Vitro and In Vivo Cellular Imaging with Green Tea Coated Water-Soluble Iron Oxide Nanocrystals

Xiao, L., Mertens, M., Wortmann, L., Kremer, S., Valldor, M., Lammers, T., et al. (2015). Enhanced In Vitro and In Vivo Cellular Imaging with Green Tea Coated Water-Soluble Iron Oxide Nanocrystals. ACS Applied Materials and Interfaces, 7(12), 6530-6540. doi:10.1021/am508404t.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Xiao, Lisong1, Author
Mertens, Marianne1, Author
Wortmann, Laura1, Author
Kremer, Silke1, Author
Valldor, Martin2, Author              
Lammers, Twan1, Author
Kiessling, Fabian1, Author
Mathur, Sanjay1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Martin Valldor, Physics of Correlated Matter, Max Planck Institute for Chemical Physics of Solids, Max Planck Society, ou_1863454              

Content

show
hide
Free keywords: -
 Abstract: Fully green and facile redox chemistry involving reduction of colloidal iron hydroxide (Fe(OH)(3)) through green tea (GT) polyphenols produced water-soluble Fe3O4 nanocrystals coated with GT extracts namely epigallocatechin gallate (EGCG) and epicatechin (EC). Electron donating polyphenols stoichiometrically reduced Fe3+ ions into Fe2+ ions resulting in the formation of magnetite (Fe3O4) nanoparticles and corresponding oxidized products (semiquinones and quinones) that simultaneously served as efficient surface chelators for the Fe3O4 nanoparticles making them dispersible and stable in water, PBS, and cell culture medium for extended time periods. As-formed iron oxide nanoparticles (2.5-6 nm) displayed high crystallinity and saturation magnetization as well as high relaxivity ratios manifested in strong contrast enhancement observed in T-2-weighted images. Potential of green tea-coated superparamagnetic iron oxide nanocrystals (SPIONs) as superior negative contrast agents was confirmed by in vitro and in vivo experiments. Primary human macrophages (J774A.1) and colon cancer cells (CT26) were chosen to assess cytotoxicity and cellular uptake of GT-, EGCGq-, and ECq-coated Fe3O4 nanoparticles, which showed high uptake efficiencies by J774A.1 and CT26 cells without any additional transfection agent. Furthermore, the in vivo accumulation characteristics of GT-coated Fe3O4 nanoparticles were similar to those observed in clinical studies of SPIONs with comparable accumulation in epidermoid cancer-xenograft bearing mice. Given their promising transport and uptake characteristics and new surface chemistry, GT-SPIONs conjugates can be applied for multimodal imaging and therapeutic applications by anchoring further functionalities.

Details

show
hide
Language(s): eng - English
 Dates: 2015-04-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000352246700022
DOI: 10.1021/am508404t
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ACS Applied Materials and Interfaces
  Abbreviation : ACS Appl. Mater. Interfaces
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 7 (12) Sequence Number: - Start / End Page: 6530 - 6540 Identifier: ISSN: 1944-8244
CoNE: https://pure.mpg.de/cone/journals/resource/1944-8244