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  Monitoring of implanted stem cell migration in vivo: A highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat

Hoehn, M., Küstermann, E., Blunk, J., Wiedermann, D., Trapp, T., Wecker, S., et al. (2002). Monitoring of implanted stem cell migration in vivo: A highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat. Proceedings of the National Academy of Sciences of the United States of America, 99(25), 16267-16272.

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 Creators:
Hoehn, Mathias1, Author           
Küstermann, Ekkehard1, Author           
Blunk, James2, Author           
Wiedermann, Dirk1, Author           
Trapp, Thorsten2, Author           
Wecker, Stefan1, Author           
Föcking, Melanie2, Author           
Arnold, Heinz3, Author
Hescheler, J.3, Author
Fleischmann, B. K.3, Author
Schwindt, Wolfram1, Author           
Bührle, Christian1, Author           
Affiliations:
1In-vivo-NMR, Research Groups, Max Planck Institute for Metabolism Research, Managing Director: Jens Brüning, Max Planck Society, ou_2149666              
2Konstantin-Alexander Hossmann, Emeriti, Max Planck Institute for Metabolism Research, Managing Director: Jens Brüning, Max Planck Society, ou_2149648              
3Max Planck Inst Neurol Res, Gleueler Str 50, D-50931 Cologne,; Germany; Max Planck Inst Neurol Res, D-50931 Cologne, Germany; Univ Cologne, Dept Neurophysiol, D-50937 Cologne, Germany, ou_persistent22              

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Free keywords: embryonic stem cells; cerebral ischemia; cell labeling
 Abstract: Copyright 2002 by the National Academy of Sciences of the United States of America, all rights reserved
 Abstract: In vivo monitoring of stem cells after grafting is essential for a better understanding of their migrational dynamics and differentiation processes and of their regeneration potential. Migration of endogenous or grafted stem cells and neurons has been described in vertebrate brain, both under normal conditions from the subventricular zone along the rostral migratory stream and under pathophysiological conditions, such as degeneration or focal cerebral ischemia. Those studies, however, relied on invasive analysis of brain sections in combination with appropriate staining techniques. Here, we demonstrate the observation of cell migration under in vivo conditions, allowing the monitoring of the cell dynamics within individual animals, and for a prolonged time. Embryonic stem (ES) cells, constitutively expressing the GFIP, were labeled by a lipofection procedure with a MRI contrast agent and implanted into rat brains. Focal cerebral ischemia had been induced 2 weeks before implantation of ES cells into the healthy, contralateral hemisphere. MRI at 78-mum isotropic spatial resolution permitted the observation of the implanted cells with high contrast against the host tissue, and was confirmed by GFP registration. During 3 weeks, cells migrated along the corpus callosum to the ventricular walls, and massively populated the borderzone of the damaged brain tissue on the hemisphere opposite to the implantation sites. Our results indicate that ES cells have high migrational dynamics, targeted to the cerebral lesion area. The imaging approach is ideally suited for the noninvasive observation of cell migration, engraftment, and morphological differentiation at high spatial and temporal resolution.

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Language(s): eng - English
 Dates: 2002-12-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 13137
ISI: 000179783400080
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Alternative Title : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Pages: - Volume / Issue: 99 (25) Sequence Number: - Start / End Page: 16267 - 16272 Identifier: ISSN: 0027-8424