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  Fluctuations between multiple EF-G-induced chimeric tRNA states during translocation on the ribosome.

Adio, S., Senyushkina, T., Peske, F., Fischer, N., Wintermeyer, W., & Rodnina, M. V. (2015). Fluctuations between multiple EF-G-induced chimeric tRNA states during translocation on the ribosome. Nature Communications, 6: 7442. doi:10.1038/ncomms8442.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-DBB8-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0651-A
Genre: Journal Article

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Adio, S., Author
Senyushkina, T.1, Author              
Peske, F.1, Author              
Fischer, N., Author
Wintermeyer, W.2, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              
2Research Group of Ribosome Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578599              

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 Abstract: The coupled translocation of transfer RNA and messenger RNA through the ribosome entails large-scale structural rearrangements, including step-wise movements of the tRNAs. Recent structural work has visualized intermediates of translocation induced by elongation factor G (EF-G) with tRNAs trapped in chimeric states with respect to 30S and 50S ribosomal subunits. The functional role of the chimeric states is not known. Here we follow the formation of translocation intermediates by single-molecule fluorescence resonance energy transfer. Using EF-G mutants, a non-hydrolysable GTP analogue, and fusidic acid, we interfere with either translocation or EF-G release from the ribosome and identify several rapidly interconverting chimeric tRNA states on the reaction pathway. EF-G engagement prevents backward transitions early in translocation and increases the fraction of ribosomes that rapidly fluctuate between hybrid, chimeric and posttranslocation states. Thus, the engagement of EF-G alters the energetics of translocation towards a flat energy landscape, thereby promoting forward tRNA movement.

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Language(s): eng - English
 Dates: 2015-06-15
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/ncomms8442
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Title: Nature Communications
Source Genre: Journal
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Pages: 11 Volume / Issue: 6 Sequence Number: 7442 Start / End Page: - Identifier: -