English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays.

Lim, J., Caen, O., Vrignon, J., Konrad, M., Taly, V., & Baret, J.-C. (2015). Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Biomicrofluidics, 9(3): 034101. doi:10.1063/1.4919415.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-1279-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1BB4-E
Genre: Journal Article

Files

show Files
hide Files
:
2156671.pdf (Publisher version), 2MB
Name:
2156671.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Lim, J.1, Author              
Caen, O., Author
Vrignon, J., Author
Konrad, M.1, Author              
Taly, V., Author
Baret, J.-C., Author
Affiliations:
1Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578612              

Content

show
hide
Free keywords: -
 Abstract: Droplet-based microfluidic technologies are powerful tools for applications requiring high-throughput, for example, in biochemistry or material sciences. Several systems have been proposed for the high-throughput production of monodisperse emulsions by parallelizing multiple droplet makers. However, these systems have two main limitations: (1) they allow the use of only a single disperse phase; (2) they are based on multiple layer microfabrication techniques. We present here a pipette-and-play solution offering the possibility of manipulating simultaneously 10 different disperse phases on a single layer device. This system allows high-throughput emulsion production using aqueous flow rates of up to 26 ml/h (>110 000 drops/s) leading to emulsions with user-defined complex chemical composition. We demonstrate the multiplex capabilities of our system by measuring the kinetics of β-galactosidase in droplets using nine different concentrations of a fluorogenic substrate.

Details

show
hide
Language(s): eng - English
 Dates: 2015-05-05
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1063/1.4919415
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biomicrofluidics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 11 Volume / Issue: 9 (3) Sequence Number: 034101 Start / End Page: - Identifier: -