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  Role of the synaptobrevin C-terminus in fusion pore formation.

Ngatchou, A. N., Kisler, K., Fang, Q., Walter, A. M., Zhao, Y., Bruns, D., et al. (2010). Role of the synaptobrevin C-terminus in fusion pore formation. Proceedings of the National Academy of Sciences of the United States of America, 107(43), 18463-18468. doi:10.1073/pnas.1006727107.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-77A8-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-8145-C
Genre: Journal Article

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 Creators:
Ngatchou, A. N., Author
Kisler, K., Author
Fang, Q., Author
Walter, A. M., Author
Zhao, Y., Author
Bruns, D., Author
Sørensen, J. B., Author
Lindau, M.1, Author              
Affiliations:
1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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Free keywords: chromaffin cell; patch clamp capacitance measurement; caged calcium; amperometry; electrochemical detector array
 Abstract: Neurotransmitter release is mediated by the SNARE proteins synaptobrevin II (sybII, also known as VAMP2), syntaxin, and SNAP-25, generating a force transfer to the membranes and inducing fusion pore formation. However, the molecular mechanism by which this force leads to opening of a fusion pore remains elusive. Here we show that the ability of sybII to support exocytosis is inhibited by addition of one or two residues to the sybII C terminus depending on their energy of transfer from water to the membrane interface, following a Boltzmann distribution. These results suggest that following stimulation, the SNARE complex pulls the C terminus of sybII deeper into the vesicle membrane. We propose that this movement disrupts the vesicular membrane continuity leading to fusion pore formation. In contrast to current models, the experiments suggest that fusion pore formation begins with molecular rearrangements at the intravesicular membrane leaflet and not between the apposed cytoplasmic leaflets.

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Language(s): eng - English
 Dates: 2010-10-26
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1006727107
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 107 (43) Sequence Number: - Start / End Page: 18463 - 18468 Identifier: -