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  F-actin and myosin II accelerate catecholamine release from chromaffin granules.

Berberian, K., Torres, A., Fang, Q., Kisler, K., & Lindau, M. (2009). F-actin and myosin II accelerate catecholamine release from chromaffin granules. Journal of Neuroscience, 29(3), 863-870. doi:10.1523/JNEUROSCI.2818-08.2009.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-77C2-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C607-8
Genre: Journal Article

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2157304.pdf (Publisher version), 325KB
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http://www.jneurosci.org/content/29/3/863.full (Publisher version)
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 Creators:
Berberian, K., Author
Torres, A., Author
Fang, Q., Author
Kisler, K., Author
Lindau, M.1, Author              
Affiliations:
1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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Free keywords: Chromaffin cells; Exocytosis; Actin; Myosin II; Amperometry; Capacitance; Fluorescence microscopy; Fusion pore
 Abstract: The roles of nonmuscle myosin II and cortical actin filaments in chromaffin granule exocytosis were studied by confocal fluorescence microscopy, amperometry, and cell-attached capacitance measurements. Fluorescence imaging indicated decreased mobility of granules near the plasma membrane following inhibition of myosin II function with blebbistatin. Slower fusion pore expansion rates and longer fusion pore lifetimes were observed after inhibition of actin polymerization using cytochalasin D. Amperometric recordings revealed increased amperometric spike half-widths without change in quantal size after either myosin II inhibition or actin disruption. These results suggest that actin and myosin II facilitate release from individual chromaffin granules by accelerating dissociation of catecholamines from the intragranular matrix possibly through generation of mechanical forces.

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Language(s): eng - English
 Dates: 2009-01-21
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1523/JNEUROSCI.2818-08.2009
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Title: Journal of Neuroscience
Source Genre: Journal
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Pages: - Volume / Issue: 29 (3) Sequence Number: - Start / End Page: 863 - 870 Identifier: -