English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-throughput in situ hybridization: Systematical production of gene expression data and beyond.

Geffers, L., & Eichele, G. (2015). High-throughput in situ hybridization: Systematical production of gene expression data and beyond. In G. Hauptmann (Ed.), In situ hybridization methods (pp. 221-245). New York: Humana Pr.; Springer. doi:10.1007/978-1-4939-2303-8_1.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-7916-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CDD6-B
Genre: Book Chapter

Files

show Files
hide Files
:
2157441.pdf (Publisher version), 797KB
 
File Permalink:
-
Name:
2157441.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Geffers, L.1, Author              
Eichele, G.1, Author              
Affiliations:
1Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society, ou_persistent34              

Content

show
hide
Free keywords: Annotation, Web databases, In situ hybridization, Functional genomics, Gene expression analysis
 Abstract: A plethora of modern-day techniques allows the detailed characterization of the transcriptome on a quantitative level. Analyses, based on techniques such as cDNA microarrays or RNA-seq (whole transcriptome shotgun sequencing), are usually genome wide in scope and readily detect small changes in gene expression levels across different biological samples. However, when it comes to spatial localization of gene expression within the context of complex tissues, traditional methods of in situ hybridization remain unparalleled with regard to their cellular resolution. Here we review methods that extend classical in situ hybridization protocols and techniques to the special needs of high-throughput (HT) studies and which can be readily scaled up to a genomic level to cover organs or even whole organisms in great detail. Moreover, we discuss suitable HT instrumentation and address postproduction issues typically arising with HT pipelines such as annotation of expression data and database organization.

Details

show
hide
Language(s): eng - English
 Dates: 2015-04-012015
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1007/978-1-4939-2303-8_1
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: In situ hybridization methods
Source Genre: Book
 Creator(s):
Hauptmann, G., Editor
Affiliations:
-
Publ. Info: New York : Humana Pr.; Springer
Pages: XX, 592 Volume / Issue: - Sequence Number: - Start / End Page: 221 - 245 Identifier: ISBN: 978-1-4939-2303-8

Source 2

show
hide
Title: Neuromethods
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 99 Sequence Number: - Start / End Page: - Identifier: -