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  Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: synthesis and evaluation in cellulo

Sim, N., Pal, R., Parker, D., Engelmann, J., Mishra, A., & Gottschalk, S. (2014). Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: synthesis and evaluation in cellulo. Organic Biomolecular Chemistry, 12(46), 9389-9404. doi:10.1039/C4OB01848F.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-7F8B-C Version Permalink: http://hdl.handle.net/21.11116/0000-0001-2217-F
Genre: Journal Article

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Sim, N, Author
Pal, R, Author
Parker, D, Author
Engelmann, J1, 2, Author              
Mishra, A, Author              
Gottschalk, S1, 2, Author              
Affiliations:
1Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497796              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: A second generation of N-methyl-D-aspartate (NMDA) receptor-targeted MRI contrast agents has been synthesised and evaluated in cellulo, based on established bicyclic NMDA receptor antagonists. Their use as responsive MR imaging probes has been evaluated in suspensions of NSC-34 cells, and one agent exhibited significant enhancements in measured longitudinal and transverse water proton relaxation rates (19 and 38% respectively; 3 T, 298 K). A biotin derivative of the lead compound was prepared and the specificity and reversibility of binding to the NMDA cell surface receptors demonstrated using confocal laser scanning microscopy. Competitive and reversible binding of glutamate to the receptors was also visualised, suggesting that the receptor-targeted approach may allow MRI to be used to monitor neuronal events associated with modulation of local glutamate concentrations.

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 Dates: 2014-12
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: DOI: 10.1039/C4OB01848F
BibTex Citekey: SimPPEMG2014
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Title: Organic Biomolecular Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 12 (46) Sequence Number: - Start / End Page: 9389 - 9404 Identifier: -