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  The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output.

Stijf-Bultsma, Y., Sommer, L., Tauber, M., Baalbaki, M., Giardoglou, P., Jones, D. R., et al. (2015). The basal transcription complex component TAF3 transduces changes in nuclear phosphoinositides into transcriptional output. Molecular Cell, 58(3), 453-467. doi:10.1016/j.molcel.2015.03.009.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-82E5-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1ADA-2
Genre: Journal Article

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Stijf-Bultsma, Y., Author
Sommer, L., Author
Tauber, M.1, Author              
Baalbaki, M., Author
Giardoglou, P., Author
Jones, D. R., Author
Gelato, K. A.1, Author              
van Pelt, J., Author
Shah, Z., Author
Rahnamoun, H., Author
Toma, C., Author
Anderson, K. E., Author
Hawkins, P., Author
Lauberth, S. M., Author
Haramis, A. P. G., Author
Hart, D., Author
Fischle, W.1, Author              
Divecha, N., Author
Affiliations:
1Research Group of Chromatin Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578604              

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 Abstract: Phosphoinositides (PI) are important signaling molecules in the nucleus that influence gene expression. However, if and how nuclear PI directly affects the transcriptional machinery is not known. We report that the lipid kinase PIP4K2B regulates nuclear PI5P and the expression of myogenic genes during myoblast differentiation. A targeted screen for PI interactors identified the PHD finger of TAF3, a TATA box binding protein-associated factor with important roles in transcription regulation, pluripotency, and differentiation. We show that the PI interaction site is distinct from the known H3K4me3 binding region of TAF3 and that PI binding modulates association of TAF3 with H3K4me3 in vitro and with chromatin in vivo. Analysis of TAF3 mutants indicates that TAF3 transduces PIP4K2B-mediated alterations in PI into changes in specific gene transcription. Our study reveals TAF3 as a direct target of nuclear PI and further illustrates the importance of basal transcription components as signal transducers.

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Language(s): eng - English
 Dates: 2015-04-092015-05-27
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.molcel.2015.03.009
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Title: Molecular Cell
Source Genre: Journal
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Pages: - Volume / Issue: 58 (3) Sequence Number: - Start / End Page: 453 - 467 Identifier: -