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  An In Vivo Metabolic Approach for Deciphering the Product Specificity of Glycerate Kinase Proves that Both E. coli's Glycerate Kinases Generate 2-Phosphoglycerate

Zelcbuch, L., Razo-Mejia, M., Herz, E., Yahav, S., Antonovsky, N., Kroytoro, H., et al. (2015). An In Vivo Metabolic Approach for Deciphering the Product Specificity of Glycerate Kinase Proves that Both E. coli's Glycerate Kinases Generate 2-Phosphoglycerate. PLoS One, 10(3): e0122957. doi:10.1371/journal.pone.0122957.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-9DA5-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-B491-3
Genre: Journal Article

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 Creators:
Zelcbuch, Lior1, Author
Razo-Mejia, Manuel1, Author
Herz, Elad1, Author
Yahav, Sagit1, Author
Antonovsky, Niv1, Author
Kroytoro, Hagar1, Author
Milo, Ron1, Author
Bar-Even, A.2, Author              
Affiliations:
1external, ou_persistent22              
2Systems and Synthetic Metabolism, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2035297              

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Free keywords: ESCHERICHIA-COLI; RECONSTRUCTION; 3-KINASE; PATHWAY; ENZYMEScience & Technology - Other Topics;
 Abstract: Apart from addressing humanity's growing demand for fuels, pharmaceuticals, plastics and other value added chemicals, metabolic engineering of microbes can serve as a powerful tool to address questions concerning the characteristics of cellular metabolism. Along these lines, we developed an in vivo metabolic strategy that conclusively identifies the product specificity of glycerate kinase. By deleting E. coli's phosphoglycerate mutases, we divide its central metabolism into an 'upper' and 'lower' metabolism, each requiring its own carbon source for the bacterium to grow. Glycerate can serve to replace the upper or lower carbon source depending on the product of glycerate kinase. Using this strategy we show that while glycerate kinase from Arabidopsis thaliana produces 3-phosphoglycerate, both E. coli's enzymes generate 2-phosphoglycerate. This strategy represents a general approach to decipher enzyme specificity under physiological conditions.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 10 (3) Sequence Number: e0122957 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850