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  A quantitative study of the Hog1 MAPK response to fluctuating osmotic stress in Saccharomyces cerevisiae

Zi, Z., Liebermeister, W., & Klipp, E. (2010). A quantitative study of the Hog1 MAPK response to fluctuating osmotic stress in Saccharomyces cerevisiae. A Quantitative Study of the Hog1 MAPK Response to Fluctuating Osmotic Stress in Saccharomyces cerevisiae, 5(3): e9522. doi:10.1371/journal.pone.0009522.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-A212-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-A213-0
Genre: Journal Article

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Zi, Zhike1, Author              
Liebermeister, Wolfram2, Author
Klipp, Edda2, Author
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1Cell Signaling Dynamics (Zhike Zi), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117284              
2Humboldt-Universität zu Berlin, Institute of Biology, Theoretical Biophysics, ou_persistent22              

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 Abstract: Background Yeast cells live in a highly fluctuating environment with respect to temperature, nutrients, and especially osmolarity. The Hog1 mitogen-activated protein kinase (MAPK) pathway is crucial for the adaption of yeast cells to external osmotic changes. Methodology/Principal Findings To better understand the osmo-adaption mechanism in the budding yeast Saccharomyces cerevisiae, we have developed a mathematical model and quantitatively investigated the Hog1 response to osmotic stress. The model agrees well with various experimental data for the Hog1 response to different types of osmotic changes. Kinetic analyses of the model indicate that budding yeast cells have evolved to protect themselves economically: while they show almost no response to fast pulse-like changes of osmolarity, they respond periodically and are well-adapted to osmotic changes with a certain frequency. To quantify the signal transduction efficiency of the osmo-adaption network, we introduced a measure of the signal response gain, which is defined as the ratio of output change integral to input (signal) change integral. Model simulations indicate that the Hog1 response gain shows bell-shaped response curves with respect to the duration of a single osmotic pulse and to the frequency of periodic square osmotic pulses, while for up-staircase (ramp) osmotic changes, the gain depends on the slope. Conclusions/Significance The model analyses suggest that budding yeast cells have selectively evolved to be optimized to some specific types of osmotic changes. In addition, our work implies that the signaling output can be dynamically controlled by fine-tuning the signal input profiles.

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Language(s): eng - English
 Dates: 2010-03-04
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0009522
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Title: A Quantitative Study of the Hog1 MAPK Response to Fluctuating Osmotic Stress in Saccharomyces cerevisiae
Source Genre: Journal
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Pages: - Volume / Issue: 5 (3) Sequence Number: e9522 Start / End Page: - Identifier: -