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  Purification of Cell Culture-Derived Influenza Virus using Continuous Simulated Moving Bed Chromatography

Kröber, T., Wolff, M., Hundt, B., Reichl, U., & Seidel-Morgenstern, A. (2011). Purification of Cell Culture-Derived Influenza Virus using Continuous Simulated Moving Bed Chromatography. Talk presented at ISPPP 2011. Alexandria, USA. 2011-10-23 - 2011-10-26.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-A388-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-A389-2
Genre: Talk

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 Creators:
Kröber, T.1, Author              
Wolff, M.1, 2, Author              
Hundt, Boris1, Author              
Reichl, U.1, 2, Author              
Seidel-Morgenstern, Andreas2, 3, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
3Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              

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 Abstract: Immunostimulating complexes (ISCOMs) are typically 40 nm spherical cage-like particles used as delivery system for vaccine antigens. They are composed of antigen, cholesterol, phospholipid and the built-in adjuvant saponin (Quil A). ISCOMs have been shown to induce strong antigen-specific cellular and humoral immune responses to various antigens in a variety of animal species. Here, the production of ISCOMs containing the hemagglutinin antigen of human influenza A virus is reported. The complexes were evaluated in terms of particle structure and size via transmission electron microscopy (TEM) and dynamic light scattering (DLS). An in house developed assay is used to characterize the in vitro stimulatory properties of ISCOMs on blood-derived human dendritic cells (DC) in comparison to inactivated influenza virus concentrates.

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Language(s): eng - English
 Dates: 2009
 Publication Status: Not specified
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Title: ISPPP 2011
Place of Event: Alexandria, USA
Start-/End Date: 2011-10-23 - 2011-10-26

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