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Abstract:
Immunostimulating complexes (ISCOMs) are typically 40 nm spherical cage-like particles used as delivery system for vaccine antigens. They are composed of antigen, cholesterol, phospholipid and the built-in adjuvant saponin (Quil A). ISCOMs have been shown to induce strong antigen-specific cellular and humoral immune responses to various antigens in a variety of animal species. Here, the production of ISCOMs containing the hemagglutinin antigen of human influenza A virus is reported. The complexes were evaluated in terms of particle structure and size via transmission electron microscopy (TEM) and dynamic light scattering (DLS). An in house developed assay is used to characterize the in vitro stimulatory properties of ISCOMs on blood-derived human dendritic cells (DC) in comparison to inactivated influenza virus concentrates.
