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  Wide-field time-correlated single photon counting (TCSPC) microscopy with time resolution below the frame exposure time

Hirvonen, L. M., Petrasek, Z., & Suhling, K. (2015). Wide-field time-correlated single photon counting (TCSPC) microscopy with time resolution below the frame exposure time. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 787(Special Issue: New Developments in Photodetection NDIP14), 1-5. doi:10.1016/j.nima.2014.09.082.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0027-A84F-B Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-0027-A852-1
Genre: Zeitschriftenartikel

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 Urheber:
Hirvonen, Liisa M.1, Autor
Petrasek, Zdenek2, Autor           
Suhling, Klaus1, Autor
Affiliations:
1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Schlagwörter: PROTEIN-PROTEIN INTERACTIONS; LIVING CELLS; LUMINESCENCEFluorescence lifetime imaging; Single photon counting; Image intensifier;
 Zusammenfassung: Fast frame rale CMOS cameras in combination with photon counting intensifiers can be used for fluorescence imaging with single photon sensitivity at kHz frame rates. We show here how the phosphor decay of the image intensifier can be exploited for accurate timing of photon arrival well below the camera exposure time This is achieved by taking ratios of the intensity of the photon events in two subsequent frames, and effectively allows wide-field TCSPC. This technique was used for measuring decays of ruthenium compound Ru(dpp) with lifetimes as low as 1 mu s with 18.5 mu s frame exposure time, including in living HeLa cells, using around 0.1 mu W excitation power. We speculate that by using an image intensifier with a faster phosphor decay to match a higher camera frame rate, photon arrival time measurements on the nanosecond time scale could well be possible. (C) 2014 Elsevier B.V. All rights reserved.

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Sprache(n): eng - English
 Datum: 2015
 Publikationsstatus: Erschienen
 Seiten: 5
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000354869900002
DOI: 10.1016/j.nima.2014.09.082
 Art des Abschluß: -

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Titel: Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS : ELSEVIER SCIENCE BV
Seiten: - Band / Heft: 787 (Special Issue: New Developments in Photodetection NDIP14) Artikelnummer: - Start- / Endseite: 1 - 5 Identifikator: ISSN: 0168-9002