English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells

Henkel, A. W., Meiri, H., Horstmann, H., Lindau, M., & Almers, W. (2000). Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells. The EMBO Journal, 19, 84-93. doi:10.1093/emboj/19.1.84.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-A4BC-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-A4BD-4
Genre: Journal Article
Alternative Title : Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells

Files

show Files
hide Files
:
EMBOJ_19_2000_84.pdf (Any fulltext), 222KB
 
File Permalink:
-
Name:
EMBOJ_19_2000_84.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Locator:
http://dx.doi.org/10.1093/emboj/19.1.84 (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Henkel, Andreas Wolfram1, Author              
Meiri, H., Author
Horstmann, Heinz2, Author              
Lindau, Manfred3, Author              
Almers, Wolfhard3, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
3Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

Content

show
hide
Free keywords: chemical oscillator; membrane capacitance; rapid freezing; staurosporine
 Abstract: Constitutive exo- and endocytic events are expected to increase and diminish the cell surface area in small spontaneous steps. Indeed, cell-attached patch-clamp measurements in resting chromaffin cells revealed spontaneous upward and downward steps in the electrical capacitance of the plasma membrane. The most frequent step size indicated cell surface changes of <0.04 ?m2, corresponding to vesicles of <110 nm diameter. Often downward steps followed upward steps within seconds, and vice versa, as if vesicles transiently opened and closed their lumen to the external space. Transient openings and closings sometimes alternated rhythmically for tens of seconds. The kinase inhibitor staurosporine dramatically increased the occurrence of such rhythmic episodes by making vesicle closure incomplete and by inhibiting fission. Staurosporine also promoted transient closures of large endocytic vesicles possibly representing remnants of secretory granules. We suggest that staurosporine blocks a late step in the endocytosis of both small and large vesicles, and that endocytosis involves a reaction cascade that can act as a chemical oscillator

Details

show
hide
Language(s): eng - English
 Dates: 2000-10-192000-11-102000-01-042000-12
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The EMBO Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 19 Sequence Number: - Start / End Page: 84 - 93 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061_1