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  A dibasic motif in the tail of a class XIV apicomplexan myosin is an essential determinant of plasma membrane localization

Hettmann, C., Herm-Götz, A., Geiter, A., Frank, B., Schwarz, E. C., Soldati, T., et al. (2000). A dibasic motif in the tail of a class XIV apicomplexan myosin is an essential determinant of plasma membrane localization. Molecular Biology of the Cell, 11(4), 1385-1400. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10749937?dopt=Abstract.

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Alternative Title : A dibasic motif in the tail of a class XIV apicomplexan myosin is an essential determinant of plasma membrane localization

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 Creators:
Hettmann, Christine, Author
Herm-Götz, Angelika, Author
Geiter, Ariane, Author
Frank, Bernd, Author
Schwarz, Eva C.1, Author              
Soldati, Thierry2, Author              
Soldati, Dominique, Author
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: Obligate intracellular parasites of the phylum Apicomplexa exhibit gliding motility, a unique form of substrate-dependent locomotion essential for host cell invasion and shown to involve the parasite actin cytoskeleton and myosin motor(s). Toxoplasma gondii has been shown to express three class XIV myosins, TgM-A, -B, and -C. We identified an additional such myosin, TgM-D, and completed the sequences of a related Plasmodium falciparum myosin, PfM-A. Despite divergent structural features, TgM-A purified from parasites bound actin in an ATP-dependent manner. Isoform-specific antibodies revealed that TgM-A and recombinant mycTgM-A were localized right beneath the plasma membrane, and subcellular fractionation indicated a tight membrane association. Recombinant TgM-D also had a peripheral although not as sharply defined localization. Truncation of their respective tail domains abolished peripheral localization and tight membrane association. Conversely, fusion of the tails to green fluorescent protein (GFP) was sufficient to confer plasma membrane localization and sedimentability. The peripheral localization of TgM-A and of the GFP-tail fusion did not depend on an intact F-actin cytoskeleton, and the GFP chimera did not localize to the plasma membrane of HeLa cells. Finally, we showed that the specific localization determinants were in the very C terminus of the TgM-A tail, and site-directed mutagenesis revealed two essential arginine residues. We discuss the evidence for a proteinaceous plasma membrane receptor and the implications for the invasion process

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Language(s): eng - English
 Dates: 1999-11-302000-01-192000-04-01
 Publication Status: Published in print
 Pages: 16
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 Rev. Type: Peer
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Title: Molecular Biology of the Cell
  Other : Mol. Biol. Cell
Source Genre: Journal
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Publ. Info: American Society for Cell Biology
Pages: - Volume / Issue: 11 (4) Sequence Number: - Start / End Page: 1385 - 1400 Identifier: ISSN: 1059-1524
CoNE: https://pure.mpg.de/cone/journals/resource/954927716372_1