English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A myosin I is involved in membrane recycling from early endosomes

Neuhaus, E. M., & Soldati, T. (2000). A myosin I is involved in membrane recycling from early endosomes. The Journal of Cell Biology, 150(5), 1013-1026. doi:10.1083/jcb.150.5.1013.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : A myosin I is involved in membrane recycling from early endosomes

Files

show Files
hide Files
:
JCellBiol_150_2000_ 1013.pdf (Any fulltext), 750KB
 
File Permalink:
-
Name:
JCellBiol_150_2000_ 1013.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-

Creators

show
hide
 Creators:
Neuhaus, Eva Maria1, Author              
Soldati, Thierry1, Author              
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

Content

show
hide
Free keywords: Dictyostelium • endocytosis • membrane recycling • myosins • sorting
 Abstract: Geometry-based mechanisms have been proposed to account for the sorting of membranes and fluid phase in the endocytic pathway, yet little is known about the involvement of the actin–myosin cytoskeleton. Here, we demonstrate that Dictyostelium discoideum myosin IB functions in the recycling of plasma membrane components from endosomes back to the cell surface. Cells lacking MyoB ( myoA 2 /B 2 , and myoB 2 cells) and wild-type cells treated with the myosin inhibitor butanedione monoxime accumulated a plasma membrane marker and biotinylated surface proteins on intracellular endocytic vacuoles. An assay based on reversible biotinylation of plasma membrane proteins demonstrated that recycling of membrane components is severely impaired in myoA/B null cells. In addition, MyoB was specifically found on magnetically purified early pinosomes. Using a rapid-freezing cryoelectron microscopy method, we observed an increased number of small vesicles tethered to relatively early endocytic vacuoles in myoA 2 /B 2 cells, but not to later endosomes and lysosomes. This accumulation of vesicles suggests that the defects in membrane recycling result from a disordered morphology of the sorting compartment

Details

show
hide
Language(s): eng - English
 Dates: 2000-02-022000-07-132000-09-05
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Cell Biology
  Other : JBC
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 150 (5) Sequence Number: - Start / End Page: 1013 - 1026 Identifier: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024_2