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  Dictyostelium myosin IK is involved in the maintenance of cortical tension and affects motility and phagocytosis

Schwarz, E. C., Neuhaus, E. M., Kistler, C., Henkel, A. W., & Soldati, T. (2000). Dictyostelium myosin IK is involved in the maintenance of cortical tension and affects motility and phagocytosis. Journal of Cell Science, 113, 621-633. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10652255.

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Genre: Journal Article
Alternative Title : Dictyostelium myosin IK is involved in the maintenance of cortical tension and affects motility and phagocytosis

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JCellSci_113_2000_621.pdf (Any fulltext), 910KB
 
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 Creators:
Schwarz, Eva C.1, Author           
Neuhaus, Eva Maria1, Author           
Kistler, Claudia2, Author           
Henkel, Andreas Wolfram2, Author           
Soldati, Thierry2, Author           
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1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: Dictyostelium discoideum myosin Ik (MyoK) is a novel type of myosin distinguished by a remarkable architecture. MyoK is related to class I myosins but lacks a cargo-binding tail domain and carries an insertion in a surface loop suggested to modulate motor velocity. This insertion shows similarity to a secondary actin-binding site present in the tail of some class I myosins, and indeed a GST-loop construct binds actin. Probably as a consequence, binding of MyoK to actin was not only ATP- but also salt-dependent. Moreover, as both binding sites reside within its motor domain and carry potential sites of regulation, MyoK might represent a new form of actin crosslinker. MyoK was distributed in the cytoplasm with a significant enrichment in dynamic regions of the cortex. Absence of MyoK resulted in a drop of cortical tension whereas overexpression led to significantly increased tension. Absence and overexpression of MyoK dramatically affected the cortical actin cytoskeleton and resulted in reduced initial rates of phagocytosis. Cells lacking MyoK showed excessive ruffling, mostly in the form of large lamellipodia, accompanied by a thicker basal actin cortex. At early stages of development, aggregation of myoK null cells was slowed due to reduced motility. Altogether, the data indicate a distinctive role for MyoK in the maintenance and dynamics of the cell cortex.

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Language(s): eng - English
 Dates: 1999-12-022000-01-312000-02-15
 Publication Status: Published in print
 Pages: 13
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 Rev. Type: Peer
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Title: Journal of Cell Science
Source Genre: Journal
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Publ. Info: Cambridge, U.K. : Co. of Biologists
Pages: - Volume / Issue: 113 Sequence Number: - Start / End Page: 621 - 633 Identifier: ISSN: 0021-9533
CoNE: https://pure.mpg.de/cone/journals/resource/954925326678