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  Electrochemical imaging of fusion pore openings by electrochemical detector arrays.

Hafez, I., Kisler, K., Berberian, K., Dernick, G., Valero, V., Yong, M. G., et al. (2005). Electrochemical imaging of fusion pore openings by electrochemical detector arrays. Proceedings of the American Academy of Sciences of the United States of America, 102(39), 13879-13884. doi:10.1073/pnas.0504098102.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-AA74-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C61E-5
Genre: Journal Article

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2167099.pdf (Publisher version), 690KB
 
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 Creators:
Hafez , I., Author
Kisler, K. , Author
Berberian, K., Author
Dernick , G., Author
Valero , V. , Author
Yong , M. G. , Author
Craighead , H. G., Author
Lindau, M.1, Author              
Affiliations:
1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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Free keywords: amperometry; exocytosis; transmitter release
 Abstract: opening of individual exocytotic fusion pores in chromaffin cells was imaged electrochemically with high time resolution. Electrochemical detector arrays that consist of four platinum microelectrodes were microfabricated on a glass coverslip. Exocytosis of single vesicles containing catecholamines from a cell positioned on top of the array is detected by the individual electrodes as a time-resolved oxidation current, reflecting the time course of arrival of catecholamine molecules at the electrode surfaces. The signals exhibit low noise and reveal foot signals indicating fusion pore formation and expansion. The position of individual release events is determined from the fraction of catecholamines recorded by the individual electrodes. Simultaneous fluorescence imaging of release of acridine orange from individual vesicles confirmed the electrochemical position assignments. This electrochemical camera provides very high time resolution, spatiotemporal localization of individual fusion pore openings and quantitative data on the flux of transmitter from individual vesicles. Analysis of the amperometric currents employing random walk simulations indicates that the time course of amperometric spikes measured near the cell surface is due to a low apparent diffusion coefficient of catecholannines near the cell surface and not due to slow dissociation from the granular matrix.

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Language(s): eng - English
 Dates: 2005-09-27
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.0504098102
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Title: Proceedings of the American Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 102 (39) Sequence Number: - Start / End Page: 13879 - 13884 Identifier: -