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  Phosphatidylinositol phosphate kinase type Iγ regulates dynamics of large dense-core vesicle fusion.

Gong, L. W., Di Paolo, G., Diaz, E., Cestra, G., Diaz, M. E., Lindau, M., et al. (2005). Phosphatidylinositol phosphate kinase type Iγ regulates dynamics of large dense-core vesicle fusion. Proceedings of the National Academy of Sciences of the United States of America, 102(14), 5204-5209. doi: 10.1073/pnas.0501412102.

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Gong, L. W., Author
Di Paolo, G., Author
Diaz, E., Author
Cestra, G., Author
Diaz, M. E., Author
Lindau, M.1, Author              
De Camilli, P., Author
Toomre, D., Author
Affiliations:
1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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 Abstract: Phosphatidylinositol-4,5-bisphosphate was proposed to be an important regulator of large dense-core vesicle exocytosis from neuroendocrine tissues. Here, we have examined the kinetics of secretion in chromaffin cells from mice lacking phosphatidylinositol phosphate kinase type Iγ, the major neuronal phosphatidylinositol-4-phosphate 5-kinase. Absence of this enzyme caused a reduction of the readily releasable vesicle pool and its refilling rate, with a small increase in morphologically docked vesicles, indicating a defect in vesicle priming. Furthermore, amperometry revealed a delay in fusion pore expansion. These results provide direct genetic evidence for a key role of phosphatidylinositol-4,5-bisphosphate synthesis in the regulation of large dense-core vesicle fusion dynamics.

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Language(s): eng - English
 Dates: 2005-04-05
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.0501412102
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 102 (14) Sequence Number: - Start / End Page: 5204 - 5209 Identifier: -