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  Compound exocytosis of granules in human neutrophils.

Lollike, K., Lindau, M., Calafat, J., & Borregaard, N. (2002). Compound exocytosis of granules in human neutrophils. Journal of Leukocyte Biology, 71(5), 973-980.

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2168093.pdf (Publisher version), 278KB
 
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Lollike, K., Author
Lindau, M.1, Author              
Calafat, J., Author
Borregaard, N., Author
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1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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Free keywords: signal transduction; patch-clamp; capacitance
 Abstract: Human neutrophils are of prime importance for the immune defense. Recent data from eosinophils and pancreatic beta cells have indicated that granules, upon exocytosis, occasionally fuse with each other in the cytosol prior to their subsequent fusion with the plasma membrane. This is termed compound exocytosis. We therefore studied exocytosis of single granules from human neutrophils by the high-resolution cell-attached patch-clamp capacitance technique. We found that 1.5% of the capacitance steps was greater than 5 fF, i.e., significantly larger than steps expected for exocytosis of single granules. The mean step size of these events was 20.5 fF, corresponding to compounds formed by at least five granules. The capacitance input from compound steps contributed more than 20% of the total capacitance increase. Electron microscopy captured morphological manifestations of transient exocytic events, confirming the functional results obtained by capacitance measurements. Compound exocytosis may be a mechanism for efficient targeting of release during exocytosis.

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Language(s): eng - English
 Dates: 2002-06
 Publication Status: Published in print
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Title: Journal of Leukocyte Biology
Source Genre: Journal
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Pages: - Volume / Issue: 71 (5) Sequence Number: - Start / End Page: 973 - 980 Identifier: -