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  Voltage dependent membrane capacitance in rat pituitary nerve terminals due to gating currents.

Kilic, G., & Lindau, M. (2001). Voltage dependent membrane capacitance in rat pituitary nerve terminals due to gating currents. Biophysical Journal, 80(3), 1220-1229.

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Kilic, G., Author
Lindau, M.1, Author              
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1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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 Abstract: We investigated the voltage dependence of membrane capacitance of pituitary nerve terminals in the whole-terminal patch-clamp configuration using a lock-in amplifier. Under conditions where secretion was abolished and voltage-gated channels were blocked or completely inactivated, changes in membrane potential still produced capacitance changes. In terminals with significant sodium currents, the membrane capacitance showed a bell-shaped dependence on membrane potential with a peak at ∼−40 mV as expected for sodium channel gating currents. The voltage-dependent part of the capacitance showed a strong correlation with the amplitude of voltage-gated Na+ currents and was markedly reduced by dibucaine, which blocks sodium channel current and gating charge movement. The frequency dependence of the voltage-dependent capacitance was consistent with sodium channel kinetics. This is the first demonstration of sodium channel gating currents in single pituitary nerve terminals. The gating currents lead to a voltage- and frequency-dependent capacitance, which can be well resolved by measurements with a lock-in amplifier. The properties of the gating currents are in excellent agreement with the properties of ionic Na+ currents of pituitary nerve terminals.

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Language(s): eng - English
 Dates: 2001-03
 Publication Status: Published online
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Title: Biophysical Journal
Source Genre: Journal
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Pages: - Volume / Issue: 80 (3) Sequence Number: - Start / End Page: 1220 - 1229 Identifier: -