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  Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells.

Henkel, A. W., Meiri, H., Horstmann, H., Lindau, M., & Almers, W. (2000). Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells. EMBO Journal, 19(1), 84-93. doi:10.1093%2Femboj%2F19.1.84.

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2168132.pdf (Publisher version), 221KB
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Henkel, A. W., Author
Meiri, H., Author
Horstmann, H., Author
Lindau, M.1, Author              
Almers, W., Author
Affiliations:
1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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 Abstract: Constitutive exo- and endocytic events are expected to increase and diminish the cell surface area in small spontaneous steps. Indeed, cell-attached patch-clamp measurements in resting chromaffin cells revealed spontaneous upward and downward steps in the electrical capacitance of the plasma membrane. The most frequent step size indicated cell surface changes of <0.04 microm(2), corresponding to vesicles of <110 nm diameter. Often downward steps followed upward steps within seconds, and vice versa, as if vesicles transiently opened and closed their lumen to the external space. Transient openings and closings sometimes alternated rhythmically for tens of seconds. The kinase inhibitor staurosporine dramatically increased the occurrence of such rhythmic episodes by making vesicle closure incomplete and by inhibiting fission. Staurosporine also promoted transient closures of large endocytic vesicles possibly representing remnants of secretory granules. We suggest that staurosporine blocks a late step in the endocytosis of both small and large vesicles, and that endocytosis involves a reaction cascade that can act as a chemical oscillator.

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Language(s): eng - English
 Dates: 2000-01-04
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1093%2Femboj%2F19.1.84
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Title: EMBO Journal
Source Genre: Journal
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Pages: - Volume / Issue: 19 (1) Sequence Number: - Start / End Page: 84 - 93 Identifier: -