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  A novel Cre recombinase reporter mouse strain facilitates selective and efficient infection of primary immune cells with adenoviral vectors

Heger, K., Kober, M., Riess, D., Drees, C., de Vries, I., Bertossi, A., et al. (2015). A novel Cre recombinase reporter mouse strain facilitates selective and efficient infection of primary immune cells with adenoviral vectors. EUROPEAN JOURNAL OF IMMUNOLOGY, 45(6), 1614-1620. doi:10.1002/eji.201545457.

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 Creators:
Heger, Klaus1, Author              
Kober, Maike1, Author              
Riess, David2, Author              
Drees, Christoph3, Author
de Vries, Ingrid3, Author
Bertossi, Arianna2, Author              
Roers, Axel3, Author
Sixt, Michael3, Author
Schmidt-Supprian, Marc1, Author              
Affiliations:
1Schmidt-Supprian, Marc / Molecular Immunology and Signaltransduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565167              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              
3external, ou_persistent22              

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Free keywords: GENE DELIVERY; EXPRESSION; RECEPTOR; LYMPHOCYTESAdenoviral infection; Conditional expression; Coxsackie; adenovirus receptor; Cre recombinase activity reporter; Knock-in mouse model;
 Abstract: Replication-deficient recombinant adenoviruses are potent vectors for the efficient transient expression of exogenous genes in resting immune cells. However, most leukocytes are refractory to efficient adenoviral transduction as they lack expression of the coxsackie/adenovirus receptor (CAR). To circumvent this obstacle, we generated the R26/CAG-CAR1(StopF) (where R26 is ROSA26 and CAG is CMV early enhancer/chicken actin promoter) knock-in mouse line. This strain allows monitoring of in situ Cre recombinase activity through expression of CAR1. Simultaneously, CAR1 expression permits selective and highly efficient adenoviral transduction of immune cell populations, such as mast cells or T cells, directly ex vivo in bulk cultures without prior cell purification or activation. Furthermore, we show that CAR1 expression dramatically improves adenoviral infection of in vitro differentiated conventional and plasmacytoid dendritic cells (DCs), basophils, mast cells, as well as Hoxb8-immortalized hematopoietic progenitor cells. This novel dual function mouse strain will hence be a valuable tool to rapidly dissect the function of specific genes in leukocyte physiology.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000355836500006
DOI: 10.1002/eji.201545457
 Degree: -

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Title: EUROPEAN JOURNAL OF IMMUNOLOGY
Source Genre: Journal
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Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 45 (6) Sequence Number: - Start / End Page: 1614 - 1620 Identifier: ISSN: 0014-2980