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  Towards instantaneous cellular level bio diagnosis: laser extraction and imaging of biological entities with conserved integrity and activity

Ren, L., Robertson, W., Reimer, R., Heinze, C., Schneider, C., Eggert, D., et al. (2015). Towards instantaneous cellular level bio diagnosis: laser extraction and imaging of biological entities with conserved integrity and activity. Nanotechnology, 26(28): 284001. doi:10.1088/0957-4484/26/28/284001.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-B4A7-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-E2A8-2
Genre: Journal Article

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 Creators:
Ren, Ling1, 2, Author              
Robertson, Wesley1, Author              
Reimer, R.3, Author
Heinze, C.4, Author
Schneider, C.3, Author
Eggert, Dennis1, Author              
Truschow, P.3, Author
Hansen, Nils-Owe1, Author              
Krötz, Peter1, 2, Author              
Zou, J.5, Author
Miller, R. J. Dwayne1, 5, Author              
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714              
3Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistraße 52, 20251 Hamburg, Germany , ou_persistent22              
4Molecular Phytopathology and Genetics, Biozentrum Klein Flottbek, Ohnhorststraße 18, 22609 Hamburg, Germany , ou_persistent22              
5Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, Ontario, M5S 3H6, Canada , ou_persistent22              

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Free keywords: laser ablation; Biodiagnosis; biomedical imaging; PACS numbers: 87.85.Xd Dynamical, regulatory, and integrative biology; 87.14.E- Proteins; 87.63.L- Visual imaging; 87.64.mk Confocal; 82.80.Ms Mass spectrometry (including SIMS, multiphoton ionization and resonance ionization mass spectrometry, MALDI); 87.15.B- Structure of biomolecules
 Abstract: The prospect for spatial imaging with mass spectroscopy at the level of the cell requires new means of cell extraction to conserve molecular structure. To this aim, we demonstrate a new laser extraction process capable of extracting intact biological entities with conserved biological function. The method is based on the recently developed picosecond infrared laser (PIRL), designed specifically to provide matrix-free extraction by selectively exciting the water vibrational modes under the condition of ultrafast desorption by impulsive vibrational excitation (DIVE). The basic concept is to extract the constituent protein structures on the fastest impulsive limit for ablation to avoid excessive thermal heating of the proteins and to use strongly resonant 1-photon conditions to avoid multiphoton ionization and degradation of the sample integrity. With various microscope imaging and biochemical analysis methods, nanoscale single protein molecules, viruses, and cells in the ablation plume are found to be morphologically and functionally identical with their corresponding controls. This method provides a new means to resolve chemical activity within cells and is amenable to subcellular imaging with near-field approaches. The most important finding is the conserved nature of the extracted biological material within the laser ablation plume, which is fully consistent with in vivo structures and characteristics.

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Language(s): eng - English
 Dates: 2014-12-112015-05-052015-06-262015-07-17
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1088/0957-4484/26/28/284001
 Degree: -

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Title: Nanotechnology
Source Genre: Journal
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Publ. Info: Bristol, UK : IOP Pub.
Pages: - Volume / Issue: 26 (28) Sequence Number: 284001 Start / End Page: - Identifier: ISSN: 0957-4484
CoNE: /journals/resource/954925577042