English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-throughput, Amplicon-Based sequencing of the CREBBP gene as a tool to develop a universal platform-independent assay

Fuellgrabe, M. W., Herrmann, D., Knecht, H., Kuenzel, S., Kneba, M., Pott, C., et al. (2015). High-throughput, Amplicon-Based sequencing of the CREBBP gene as a tool to develop a universal platform-independent assay. PLoS One, 0129195. doi:10.1371/journal.pone.0129195.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-BA30-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-BA31-9
Genre: Journal Article

Files

show Files
hide Files
:
Fuellgrabe_et_al_2015.pdf (Publisher version), 2MB
Name:
Fuellgrabe_et_al_2015.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Fuellgrabe, Marc W., Author
Herrmann, Dietrich, Author
Knecht, Henrik, Author
Kuenzel, Sven1, Author              
Kneba, Michael, Author
Pott, Christiane, Author
Brüggemann, Monika, Author
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              

Content

show
hide
Free keywords: -
 Abstract: High-throughput sequencing technologies are widely used to analyse genomic variants or rare mutational events in different fields of genomic research, with a fast development of new or adapted platforms and technologies, enabling amplicon-based analysis of single target genes or even whole genome sequencing within a short period of time. Each sequencing platform is characterized by well-defined types of errors, resulting from different steps in the sequencing workflow. Here we describe a universal method to prepare amplicon libraries that can be used for sequencing on different high-throughput sequencing platforms. We have sequenced distinct exons of the CREB binding protein (CREBBP) gene and analysed the output resulting from three major deep-sequencing platforms. platform-specific errors were adjusted according to the result of sequence analysis from the remaining platforms. Additionally, bioinformatic methods are described to determine platform dependent errors. Summarizing the results we present a platform-independent cost-efficient and timesaving method that can be used as an alternative to commercially available sample-preparation kits.

Details

show
hide
Language(s): eng - English
 Dates: 2015-03-092015-04-072015-06-09
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0129195
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Francisco, CA : Public Library of Science
Pages: 12 S. Volume / Issue: - Sequence Number: 0129195 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850