English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria.

Melin, J., Kilisch, M., Neumann, P., Lytovchenko, O., Gomkale, R., Schendzielorz, A., et al. (2015). A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria. Biochimica et Biophysica Acta-Molecular Cell Research, 1853(8), 1850-1859. doi:10.1016/j.bbamcr.2015.04.021.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-BA93-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-58FE-D
Genre: Journal Article

Files

show Files
hide Files
:
2170687.pdf (Publisher version), 2MB
Name:
2170687.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Melin, J., Author
Kilisch, M., Author
Neumann, P., Author
Lytovchenko, O., Author
Gomkale, R., Author
Schendzielorz, A., Author
Schmidt, B., Author
Liepold, T., Author
Ficner, R., Author
Jahn, O., Author
Rehling, P.1, Author              
Schulz, C., Author
Affiliations:
1Max Planck Fellow Peter Rehling, ou_1298545              

Content

show
hide
Free keywords: Tom70; Mitochondria; Protein import; Presequence
 Abstract: The translocase of the outer mitochondrial membrane (TOM complex) is the general entry gate into mitochondria for almost all imported proteins. A variety of specific receptors allow the TOM complex to recognize targeting signals of various precursor proteins that are transported along different import pathways. Aside from the well-characterized presequence receptors Tom20 and Tom22 a third TOM receptor, Tom70, binds proteins of the carrier family containing multiple transmembrane segments. Here we demonstrate that Tom70 directly binds to presequence peptides using a dedicated groove. A single point mutation in the cavity of this pocket (M551R) reduces the presequence binding affinity of Tom70 ten-fold and selectively impairs import of the presequence-containing precursor Mdl1 but not the ADP/ATP carrier (MC). Hence Tom70 contributes to the presequence import pathway by recognition of the targeting signal of the Mdl1 precursor.

Details

show
hide
Language(s): eng - English
 Dates: 2015-05-072015-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.bbamcr.2015.04.021
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biochimica et Biophysica Acta-Molecular Cell Research
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1853 (8) Sequence Number: - Start / End Page: 1850 - 1859 Identifier: -