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  Cortical actin networks induce spatio-temporal confinement of phospholipids in the plasma membrane - a minimally invasive investigation by STED-FCS.

Andrade, D. M., Clausen, M. P., Keller, J., Müller, V., Wu, C., Bear, J. E., et al. (2015). Cortical actin networks induce spatio-temporal confinement of phospholipids in the plasma membrane - a minimally invasive investigation by STED-FCS. Scientific Reports, 5: 11454. doi:10.1038/srep11454.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-BB21-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-6DFE-2
Genre: Journal Article

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 Creators:
Andrade, D. M., Author
Clausen, M. P., Author
Keller, J.1, Author              
Müller, V.1, Author              
Wu, C., Author
Bear, J. E., Author
Hell, S. W.1, Author              
Lagerholm, B. C., Author
Eggeling, C.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Important discoveries in the last decades have changed our view of the plasma membrane organisation. Specifically, the cortical cytoskeleton has emerged as a key modulator of the lateral diffusion of membrane proteins. Cytoskeleton-dependent compartmentalised lipid diffusion has been proposed, but this concept remains controversial because this phenomenon has thus far only been observed with artefact-prone probes in combination with a single technique: single particle tracking. In this paper, we report the first direct observation of compartmentalised phospholipid diffusion in the plasma membrane of living cells using a minimally invasive, fluorescent dye labelled lipid analogue. These observations were made using optical STED nanoscopy in combination with fluorescence correlation spectroscopy (STED-FCS), a technique which allows the study of membrane dynamics on a sub-millisecond time-scale and with a spatial resolution of down to 40nm. Specifically, we find that compartmentalised phospholipid diffusion depends on the cortical actin cytoskeleton, and that this constrained diffusion is directly dependent on the F-actin branching nucleator Arp2/3. These findings provide solid evidence that the Arp2/3-dependent cortical actin cytoskeleton plays a pivotal role in the dynamic organisation of the plasma membrane, potentially regulating fundamental cellular processes.

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Language(s): eng - English
 Dates: 2015-06-29
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/srep11454
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Title: Scientific Reports
Source Genre: Journal
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Pages: 12 Volume / Issue: 5 Sequence Number: 11454 Start / End Page: - Identifier: -