English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Bacterial lipopolysaccharides form physically cross-linked, two-dimensional gels in the presence of divalent cations

Herrmann, M., Schneck, E., Gutsmann, T., Brandenburg, K., & Tanaka, M. (2015). Bacterial lipopolysaccharides form physically cross-linked, two-dimensional gels in the presence of divalent cations. Soft Matter. doi:10.1039/C5SM01002K.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-BD7F-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-BD80-E
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Herrmann, Moritz, Author
Schneck, Emanuel1, Author              
Gutsmann, Thomas, Author
Brandenburg, Klaus, Author
Tanaka, Motomu, Author
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: We established a bacterial membrane model with monolayers of bacterial lipopolysaccharides (LPS Re and LPS Ra) and quantified their viscoelastic properties by using an interfacial stress rheometer coupled to a Langmuir film balance. LPS Re monolayers exhibited purely viscous behaviour in the absence of calcium ions,} while the same monolayers underwent a viscous-to-elastic transition upon compression in the presence of Ca2+. Our results demonstrated for the first time that LPSs in bacterial outer membranes can form two-dimensional elastic networks in the presence of Ca2+. Different from LPS Re monolayers{,} the LPS Ra monolayers showed a very similar rheological transition both in the presence and absence of Ca2+{,} suggesting that longer saccharide chains can form 2D physical gels even in the absence of Ca2+. By exposure of the monolayers to the antimicrobial peptide protamine{, we could directly monitor the differences in resistance of bacterial membranes according to the presence of calcium.

Details

show
hide
Language(s):
 Dates: 2015-07-02
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1039/C5SM01002K
BibTex Citekey: C5SM01002K
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Soft Matter
  Abbreviation : Soft Matter
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1744-683X
CoNE: https://pure.mpg.de/cone/journals/resource/1744-683X